Cold Spring Harb Protoc. 2020 Dec 1;2020(12):2020/12/pdb.prot100420. doi: 10.1101/pdb.prot100420.
Bands of radioactive DNA separated by polyacrylamide gel electrophoresis may be detected by autoradiography or phosphorimaging. Analytical polyacrylamide gels containing radioactive DNA are usually fixed and dried before autoradiography. However, if bands of radioactive DNA are to be recovered from the gel, the gel should generally not be fixed or dried.
经聚丙烯酰胺凝胶电泳分离的放射性 DNA 带可用放射自显影或磷光成像来检测。含有放射性 DNA 的分析性聚丙烯酰胺凝胶通常在放射自显影之前进行固定和干燥。然而，如果要从凝胶中回收放射性 DNA 带，则通常不应固定或干燥凝胶。
