人乳头瘤病毒16 E6和E7癌基因对人喉鳞状细胞癌细胞系增殖和分化的影响

[Effects of human papillomavirus 16 E6 and E7 oncogene on the proliferation and differentiation of human laryngeal squamous cell carcinoma cell line].

作者信息

Cai Ping, Wu Zhan-yuan, Li Jin-rong

机构信息

Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School of Stomatology, Wuhan University, Wuhan 430079, China.

出版信息

Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2007 Oct;42(10):774-8.

PMID:18229591

Abstract

OBJECTIVE

To investigate the correlation between HPV16 and the development of laryngeal squamous cell carcinoma by studying the effects of HPV16 E6 and E7 oncogene on the proliferation and differentiation of human laryngeal squamous carcinoma cell line.

METHODS

Cationic phosphonolipid was used to transfect pLXSN16E6E7 into Lscc-02 high differentiation laryngeal squamous cell carcinoma cell line, the cells transfected by the vector pLXSN and Lscc-02 cells served as control groups. The changes of proliferation and differentiation were measured in vitro and in vivo.

RESULTS

The proliferation was quicker in experimental group. The average A ratio reached to 2.96, which was higher than 1.90 and 1.85 of control groups. The clone forming rate was 23.6% in experimental group, which was higher than 12.7% and 12.0% of control groups. Serum-dependent became lower in experimental group. The Ki-67 positive expression rate was markedly increased and cytokeratin 13 positive expression rate was markedly decreased in experimental group as compared with control groups. The Ki-67 positive expression rates were 93.8%, 80.7% and 79.2% respectively. The cytokeratin 13 positive expression rate were 80.9%, 91.0% and 93.7% respectively. The latent period and internal double time of transplant tumor were short in experimental group compared with control groups. The latent period were 19 d, 28 d and 30 d respectively. The internal double time of transplant tumor was 2.15 d, 3.28 d and 3.47 d respectively. The volume of transplant tumor was smaller in the same period in experimental group. The cells of transplant tumor in experimental group showed an image of small size, great allotype and a trend of low differentiation.

CONCLUSIONS

HPV16 E6 and E7 can promote the proliferation and inhibit the differentiation of Lscc-02 laryngeal squamous cell carcinoma cells which suggest HPV16 may play an important role in the development of laryngeal squamous cell carcinoma.

摘要

目的

通过研究人乳头瘤病毒16型（HPV16）E6和E7癌基因对人喉鳞状癌细胞系增殖和分化的影响，探讨HPV16与喉鳞状细胞癌发生发展的相关性。

方法

采用阳离子磷脂将pLXSN16E6E7转染入Lscc-02高分化喉鳞状癌细胞系，以转染载体pLXSN的细胞及Lscc-02细胞作为对照组。在体外和体内检测增殖和分化的变化。

结果

实验组增殖较快。平均A值达到2.96，高于对照组的1.90和1.85。实验组克隆形成率为23.6%，高于对照组的12.7%和12.0%。实验组血清依赖性降低。与对照组相比，实验组Ki-67阳性表达率明显升高，细胞角蛋白13阳性表达率明显降低。Ki-67阳性表达率分别为93.8%、80.7%和79.2%。细胞角蛋白13阳性表达率分别为80.9%、91.0%和93.7%。与对照组相比，实验组移植瘤的潜伏期和倍增时间较短。潜伏期分别为19天、28天和30天。移植瘤倍增时间分别为2.15天、3.28天和3.47天。实验组同期移植瘤体积较小。实验组移植瘤细胞呈现体积小、异型性大及低分化趋势。