宫颈癌组织中人类乳头瘤病毒16E6基因突变

[Human papillomavirus 16E6 gene mutation in cervical carcinoma tissues].

作者信息

Sun Feng, Ha Xiao-Qin, Lv Tong-De, Xing Chuan-Ping, Liu Bin, Cao Xiao-Zhe

机构信息

Department of Pathology, Lanzhou General Hospital of PLA, Lanzhou, Gansu, China.

出版信息

Ai Zheng. 2009 May;28(5):478-82.

PMID:19624874

Abstract

BACKGROUND AND OBJECTIVE

Human papillomavirus (HPV) 16 is the most common type of high-risk human HPVs. HPV16 E6 gene and its specific mutations are considered as risk factors causing cervical carcinoma (CC). This study was to investigate HPV16 E6 mutations in Lanzhou region and explore the relationship between HPV16 E6 mutations and the development of CC.

METHODS

Tissue DNA was extracted from 23 patients operated on for CC and five normal cervical controls. The partial sequence of the HPV16 E6 gene (nucleotide 201-523) was amplified by PCR from the tissue DNA extracted from the samples. PCR fragments were sequenced and analyzed.

RESULTS

The positive rates of HPV16 E6 in five normal cervical and 23 CC tissues were 0 (0/5) and 82.61% (19/23), respectively. Prototype HPV16E6 gene was found in six cases (33.33%) while mutation in the E6 gene was detected in 12 cases (66.67%), among which a 350G mutation was found in 11 cases (61.11%). Moreover, a 249G mutation was identified in one CC case (5.56%).

CONCLUSIONS

There is a high HPV infection rate in CC tissues in Lanzhou region, and most of the HPV16E6 are mutated.

摘要

背景与目的

人乳头瘤病毒（HPV）16型是高危型人乳头瘤病毒中最常见的类型。HPV16 E6基因及其特定突变被认为是导致宫颈癌（CC）的危险因素。本研究旨在调查兰州地区HPV16 E6突变情况，并探讨HPV16 E6突变与宫颈癌发生发展的关系。

方法

从23例接受宫颈癌手术的患者及5例正常宫颈对照者中提取组织DNA。从样本提取的组织DNA中通过聚合酶链反应（PCR）扩增HPV16 E6基因的部分序列（核苷酸201 - 523）。对PCR片段进行测序和分析。

结果

5例正常宫颈组织和23例宫颈癌组织中HPV16 E6的阳性率分别为0（0/5）和82.61%（19/23）。6例（33.33%）发现HPV16E6基因原型，12例（66.67%）检测到E6基因有突变，其中11例（61.11%）发现350G突变。此外，在1例宫颈癌病例（5.56%）中鉴定出249G突变。