Mayevsky A
Brain Res. 1976 Aug 27;113(2):327-38. doi: 10.1016/0006-8993(76)90944-6.
Using a time-sharing fluorometer-reflectometer, pyridine nucleotide (NADH) and flavoprotein (Fp) fluorescence, as well as reflected light at the excitation wavelength, were measured and correlated with the electrical activity of an awake cerebral cortex. Exposure of the rat to a nitrogen atmosphere (anoxia) led to an increase in signals representing the reduction of pyridine nucleotides and flavins, with very similar kinetics. Inducement of partial ischemia by bilateral carotid artery ligation led to an increase in NADH, accompanied by a very small effect on the electrical activity (ECoG). In most animals, 2-3h after ligation, the ECoG became flat or depressed. Exposure of this ischemic cerebral cortex to KC1 solution caused depression of the electrical activity without metabolic response probably due to the limitation of oxygen supply. The metabolic state of an awake cerebral cortex was identified by exposing the brain to various levels of oxygen, epileptoform activity, spreading depression, hyperbaric pressure of oxygen and an uncoupler. From our results we conclude that the awake cerebral cortex is close to the resting state, state 4, rather than to the active state, state 3.
使用分时荧光计-反射计，测量了吡啶核苷酸（NADH）和黄素蛋白（Fp）的荧光以及激发波长下的反射光，并将其与清醒大脑皮层的电活动相关联。将大鼠暴露于氮气环境（缺氧）会导致代表吡啶核苷酸和黄素还原的信号增加，其动力学非常相似。双侧颈动脉结扎诱导局部缺血会导致NADH增加，同时对电活动（脑电图）的影响非常小。在大多数动物中，结扎后2 - 3小时，脑电图变得平坦或降低。将这种缺血性大脑皮层暴露于氯化钾溶液会导致电活动降低，且无代谢反应，这可能是由于氧气供应受限所致。通过将大脑暴露于不同水平的氧气、癫痫样活动、扩散性抑制、高压氧和一种解偶联剂，确定了清醒大脑皮层的代谢状态。从我们的结果可以得出结论，清醒大脑皮层接近静息状态，即状态4，而不是活跃状态，即状态3。
