Barlow C H, Harden W R, Harken A H, Simson M B, Haselgrove J C, Chance B, O'Connor M, Austin G
Crit Care Med. 1979 Sep;7(9):402-6. doi: 10.1097/00003246-197909000-00011.
Fluorescence techniques may be utilized to map changes in the distribution of mitochondrial redox states in heart and brain during ischemic or hypoxic stress. The basis of these techniques is the intrinsic fluorescence of reduced NADH and oxidized flavoprotein in mitochondria which respond to changes in critical oxygen supply. Ischemic areas in rabbit hearts induced by coronary ligation were detected and mapped based on the increase in NADH fluorescence in the ischemic zone. The width of the jeopardized normoxic tissue surrounding the ischemic area (less than 50--350 mu) was measured by combination of fluorescein angiography and NADH fluorescence. Areas of increased NADH fluorescence in gerbil brains after carotid artery ligation or induction of spreading depression were mapped in a similar manner. Intraoperative monitoring of flavoprotein fluorescence from human cerebral cortex after superficial temporal artery middle cerebral artery (STA-MCA) anastomoses demonstrated increased rates of cortical oxidative metabolism after the surgical procedures.
荧光技术可用于描绘缺血或缺氧应激期间心脏和大脑中线粒体氧化还原状态分布的变化。这些技术的基础是线粒体中还原型烟酰胺腺嘌呤二核苷酸（NADH）和氧化型黄素蛋白的固有荧光，它们对关键氧供应的变化做出反应。基于缺血区NADH荧光的增加，检测并绘制了冠状动脉结扎诱导的兔心脏缺血区域。通过荧光素血管造影和NADH荧光相结合的方法，测量了缺血区域周围受威胁的常氧组织的宽度（小于50-350微米）。以类似的方式绘制了沙鼠大脑在颈动脉结扎或诱导扩散性抑制后NADH荧光增加的区域。颞浅动脉-大脑中动脉（STA-MCA）吻合术后对人大脑皮层黄素蛋白荧光的术中监测表明，手术后皮层氧化代谢率增加。
