Cruess R L, Hong K C
Calcif Tissue Res. 1976 Jun 14;20(3):317-20. doi: 10.1007/BF02546419.
Rats were injected with 17 beta-estradiol benzoate intramuscularly and the collagenolytic activity of the rat bones was assayed by measuring the degradation of purified reconstituted 3H-proline- and 3H-hydroxyproline-labeled collagen. A diminution of the collagenolytic activity which could be recovered from the culture medium of estrogen-treated rat bone was observed. However, the addition of estrogen to the enzyme using the same assay revealed no diminution of collagenolytic activity. These results suggest that the diminution of collagenolytic activity in the culture medium of estrogen-treated rat bone is due to an alteration in the production or activation of collagenolytic activity.
给大鼠肌肉注射苯甲酸雌二醇，通过测量纯化的重组3H-脯氨酸和3H-羟脯氨酸标记的胶原蛋白的降解来测定大鼠骨骼的胶原酶活性。观察到从雌激素处理的大鼠骨骼培养基中可恢复的胶原酶活性降低。然而，使用相同的测定方法向酶中添加雌激素并未显示胶原酶活性降低。这些结果表明，雌激素处理的大鼠骨骼培养基中胶原酶活性的降低是由于胶原酶活性的产生或激活发生了改变。
