Langeland N
Acta Orthop Scand. 1977;48(3):266-72. doi: 10.3109/17453677708988766.
Surviving bone pieces from the femoral and tibial metaphyses of young castrated female rats have been incubated for 6 hours with concentrations of oestradiol-17-beta ranging from 10(-9) M to 3 x 10(-5) M, and the in vitro collagen metabolism studied. The addition of oestradiol did not produce any change in the resorption patterns of bone collagen, expressed as release of hydroxyproline to the medium. Parathyroid hormone in the incubation medium increased the resorption and decreased the incorporation rate insignificantly. Addition of both parathyroid hormone and oestradiol to the incubation medium produced a significant increase in resorption and a decrease in incorporation rate as compared with the control incubations. The bone pieces incubated with oestradiol-17-beta 3 x 10(-5) synthesized and incorporated significantly less hydroxyproline than the control bone pieces. This is, however, a very high concentration of the hormone and the physiological significance of the observation is doubtful.
将年轻去势雌性大鼠股骨和胫骨干骺端的存活骨碎片，与浓度范围为10(-9)M至3×10(-5)M的17-β-雌二醇一起孵育6小时，并研究体外胶原代谢。添加雌二醇并未使骨胶原的吸收模式产生任何变化，骨胶原的吸收模式以羟脯氨酸向培养基中的释放来表示。孵育培养基中的甲状旁腺激素增加了吸收，并使掺入率略有降低。与对照孵育相比，向孵育培养基中同时添加甲状旁腺激素和雌二醇，导致吸收显著增加，掺入率降低。与对照骨碎片相比，用3×10(-5)的17-β-雌二醇孵育的骨碎片合成和掺入的羟脯氨酸明显更少。然而，这是该激素的一个非常高的浓度，该观察结果的生理意义值得怀疑。
