Bai Ying, Kosoy Michael, Martin Andrew, Ray Chris, Sheff Kelly, Chalcraft Linda, Collinge Sharon K
Department of Ecology and Evolutionary Biology, University of Colorado, Boulder, CO, USA.
Vector Borne Zoonotic Dis. 2008 Spring;8(1):1-5. doi: 10.1089/vbz.2007.0136.
Thirty bartonella strains were isolated from the blood of black-tailed prairie dogs (Cynomys ludovicianus) from Boulder County, Colorado, USA. The bacteria appeared as small, fastidious, aerobic, Gram-negative rods. The partial sequences of the citrate synthase gene (gltA) demonstrated five unique genetic variants. Phylogenetic analysis based on sequences of gltA, 16S rRNA, rpoB, ftsZ, and ribC showed that the black-tailed prairie dog-related Bartonella variants comprise a distinct monophyletic clade that is closely related to Bartonella washoensis, a species isolated from a human patient and subsequently from ground squirrels. These variants, however, are grouped together in 100% of the bootstrapped trees. These variants were not found in other small mammals trapped during the same study, showing some evidence of host specificity. We believe that the group being described here is typical of the black-tailed prairie dog. We propose to name the bacteria Candidatus Bartonella washoensis subsp. cynomysii. The type strain is CL8606co(T)(=ATCC BAA-1342(T) = CCUG 53213(T)), which is the representative isolate of the dominant variant of the characterized group.
从美国科罗拉多州博尔德县的黑尾草原犬鼠（Cynomys ludovicianus）血液中分离出30株巴尔通体菌株。这些细菌呈小的、苛求营养的、需氧的革兰氏阴性杆菌。柠檬酸合酶基因（gltA）的部分序列显示出5种独特的遗传变异。基于gltA、16S rRNA、rpoB、ftsZ和ribC序列的系统发育分析表明，与黑尾草原犬鼠相关的巴尔通体变异体构成一个独特的单系分支，与从一名人类患者以及随后从地松鼠中分离出的沃肖巴尔通体（Bartonella washoensis）密切相关。然而，在100%的自展树中，这些变异体被归为一类。在同一研究中捕获的其他小型哺乳动物中未发现这些变异体，这显示出一定的宿主特异性证据。我们认为这里描述的菌群是黑尾草原犬鼠的典型菌群。我们提议将这些细菌命名为“暂定沃肖巴尔通体犬鼠亚种（Candidatus Bartonella washoensis subsp. cynomysii）”。模式菌株是CL8606co(T)(=ATCC BAA - 1342(T) = CCUG 53213(T))，它是已鉴定菌群中优势变异体的代表性分离株。
