Prevalence and genetic diversity of rodent-associated in Hulunbuir border regions, China.

spp. are globally distributed gram-negative facultative intracellular bacteria that infect a wide range of hosts. Rodents are natural reservoirs of many species, some of which are also pathogenic to humans. The rapid development of transportation and tourism has highlighted the risk of transmission to humans. Thus, it is essential to maintain surveillance of spp. infections in rodents. In China, spp. infections have been monitored in various areas; however, these have not included the Hulunbuir border regions. In the present study, we monitored the prevalence and genetics of rodent-associated spp. in the Hulunbuir border regions. Eleven rodent species were captured at five ports. Eight species were confirmed as -positive using quantitative PCR assay, with an overall positivity rate of 20.05 %. was the predominant rodent species captured for detection Sequencing and phylogenetic analysis (using the maximum likelihood method) revealed the presence of three species in these rodents, including two pathogenic to humans, namely, and . was the predominant species identified in the rodents. Taken together, these results highlight the prevalence and genetic diversity of spp. in rodents in the Hulunbuir border regions, indicating the need for risk assessment of human spillover.