Neira Oviedo M, Vanekeris L, Corena-McLeod M D P, Linser P J
The Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, FL 32080, USA.
Insect Mol Biol. 2008 Feb;17(1):61-72. doi: 10.1111/j.1365-2583.2008.00779.x.
The alimentary canal of the larval mosquito displays a considerable degree of physiological compartmentalization among its different anatomical sub-divisions (gastric caeca, anterior midgut, posterior midgut and hindgut). We performed a comparative microarray analysis in order to identify transcripts which are particularly enriched in each gut section. Based on the available annotation of the selected transcripts, we suggest that the metabolism and absorption of proteins and carbohydrates takes place mainly in the gastric caeca and posterior midgut, whereas the anterior midgut specializes in the metabolism and absorption of lipids. Transcripts encoding antimicrobial peptides were found to be enriched in the gastric caeca, and a high enrichment of transcripts associated with enzymes involved in xenobiotic detoxification was found in the anterior midgut. Furthermore, our data support the notion that the region encompassing the hindgut and Malpighian tubes plays important roles in avoiding the excretion of nutrients, as well as in xenobiotic detoxification.
幼虫蚊子的消化道在其不同的解剖亚区（胃盲囊、中肠前部、中肠后部和后肠）之间表现出相当程度的生理分隔。我们进行了一项比较微阵列分析，以鉴定在每个肠道部分特别富集的转录本。基于所选转录本的现有注释，我们认为蛋白质和碳水化合物的代谢和吸收主要发生在胃盲囊和中肠后部，而中肠前部专门负责脂质的代谢和吸收。发现编码抗菌肽的转录本在胃盲囊中富集，并且在前部中肠中发现与参与异生物质解毒的酶相关的转录本高度富集。此外，我们的数据支持这样一种观点，即包含后肠和马氏管的区域在避免营养物质排泄以及异生物质解毒方面发挥着重要作用。
