一种用于炭疽芽孢杆菌的高分辨率四基因座多重单核苷酸重复序列（SNR）基因分型系统。
A high resolution four-locus multiplex single nucleotide repeat (SNR) genotyping system in Bacillus anthracis.
作者信息
Kenefic L J, Beaudry J, Trim C, Huynh L, Zanecki S, Matthews M, Schupp J, Van Ert M, Keim P
机构信息
Center for Microbial Genetics and Genomics, Northern Arizona University, Flagstaff, AZ 86011-5640, United States.
出版信息
J Microbiol Methods. 2008 Jun;73(3):269-72. doi: 10.1016/j.mimet.2007.11.014. Epub 2007 Nov 22.
The allelic identities of Single Nucleotide Repeat (SNR) markers in Bacillus anthracis are typically ascertained by DNA sequencing through the direct repeat. Here we describe a reproducible method for genotyping closely related isolates by using four SNR loci in a multiplex-PCR capillary electrophoresis system amenable to high-throughput analysis.
炭疽芽孢杆菌中单核苷酸重复（SNR）标记的等位基因身份通常通过对直接重复序列进行DNA测序来确定。在此，我们描述了一种可重复的方法，用于在适用于高通量分析的多重PCR毛细管电泳系统中，利用四个SNR基因座对密切相关的分离株进行基因分型。
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