Wang Na, Zheng Quanhui, Zhang Jin-San, Zhao Yong
Transplantation Biology Research Division, State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Datun Road, Beijing 100101, China.
J Mol Histol. 2008 Jun;39(3):295-302. doi: 10.1007/s10735-008-9165-2. Epub 2008 Feb 1.
Zinc finger protein (Zfp) 185 is a mouse protein containing a Lin-l1, Isl-1 and Mec-3 (LIM) domains at its C-terminus. It was recognized by comparing the genome sequence between humans and mice in 1997. In this study, we cloned the full-length Zfp185 by means of RACE and RT-PCR. Zfp185 may be closely associated with F-actin in cells as determined by a confocal microscopy. With a series of deletants of Zfp185 and GST-pull-down assay, we determined that N-terminus region (1-144) but not the LIM domain at C-terminus of Zfp185 protein was essential and sufficient to bind to F-actin cytoskeleton. Thus, our data offered evidence for the association of mouse Zfp185 with F-actin, which supports the potential role of Zfp185 in cell fundamental activity.
锌指蛋白（Zfp）185是一种小鼠蛋白，其C端含有一个Lin-l1、Isl-1和Mec-3（LIM）结构域。它是在1997年通过比较人类和小鼠的基因组序列而被识别出来的。在本研究中，我们通过RACE和RT-PCR克隆了全长Zfp185。通过共聚焦显微镜观察确定，Zfp185可能在细胞中与F-肌动蛋白密切相关。利用一系列Zfp185缺失体和GST下拉实验，我们确定Zfp185蛋白的N端区域（1-144）而非C端的LIM结构域对于结合F-肌动蛋白细胞骨架是必需且充分的。因此，我们的数据为小鼠Zfp185与F-肌动蛋白的关联提供了证据，这支持了Zfp185在细胞基本活动中的潜在作用。
