Ramírez-Farías Carlett, Madrigal-Santillán Eduardo, Gutiérrez-Salinas José, Rodríguez-Sánchez Nidia, Martínez-Cruz Maricela, Valle-Jones Ilse, Gramlich-Martínez Ingrid, Hernández-Ceruelos Alejandra, Morales-Gonzaléz José A
Unidad de Genética de la Nutrición, Instituto Nacional de Pediatría, México.
World J Gastroenterol. 2008 Feb 14;14(6):899-907. doi: 10.3748/wjg.14.899.
To investigate the effects of vitamins (A, C and E) on liver injury induced by ethanol administration during liver regeneration in rats.
Male Wistar rats subjected to 70% partial hepatectomy were divided into five groups (groups 1-5). During the experiment, animals of Group 1 drank only water. The other four groups (2-5) drank 30 mL of ethanol/L of water. Group 3 additionally received vitamin A, those of group 4 vitamin C and those of group 5 received vitamin E. Subsequently serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), albumin and bilirubin were measured colorimetrically. Lipid peroxidation (thiobarbituric-acid reactive substances, TBARS) both in plasma and liver was measured, as well as liver mass gain assessment and total DNA.
Compared with sham group, serum AST and ALT increased significantly under ethanol treatment (43% and 93%, respectively, with P < 0.05). Vitamin C and vitamin E treatment attenuated the ethanol-induced increases in ALT and AST activity. Ethanol treatment also decreased serum albumin concentration compared to sham group (3.1 +/- 0.4 g/dL vs 4.5 +/- 0.2 g/dL; P < 0.05). During liver regeneration vitamins C and E significantly ameliorated liver injury for ethanol administration in hepatic lipid peroxidation (4.92 nmol/mg and 4.25 nmol/mg vs 14.78 nmol/mg, respectively, with P < 0.05). In association with hepatic injury, ethanol administration caused a significant increase in both hepatic and plasma lipid peroxidation. Vitamins (C and E) treatment attenuated hepatic and plasma lipid peroxidation.
Vitamins C and E protect against liver injury and dysfunction, attenuate lipid peroxidation, and thus appear to be significantly more effective than vitamin A against ethanol-mediated toxic effects during liver regeneration.
研究维生素（A、C和E）对大鼠肝再生过程中乙醇诱导的肝损伤的影响。
将接受70%部分肝切除术的雄性Wistar大鼠分为五组（1 - 5组）。实验期间，第1组动物只饮水。其他四组（2 - 5组）饮用含30 mL乙醇/L水的溶液。第3组额外给予维生素A，第4组给予维生素C，第5组给予维生素E。随后采用比色法测定血清丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、白蛋白和胆红素。测定血浆和肝脏中的脂质过氧化（硫代巴比妥酸反应性物质，TBARS），以及肝脏质量增加评估和总DNA。
与假手术组相比，乙醇处理后血清AST和ALT显著升高（分别升高43%和93%，P < 0.05）。维生素C和维生素E处理减轻了乙醇诱导的ALT和AST活性升高。与假手术组相比，乙醇处理还降低了血清白蛋白浓度（3.1±0.4 g/dL对4.5±0.2 g/dL；P < 0.05）。在肝再生过程中，维生素C和E显著改善了乙醇给药所致的肝损伤，表现为肝脂质过氧化减轻（分别为4.92 nmol/mg和4.25 nmol/mg，而乙醇组为14.78 nmol/mg，P < 0.05）。与肝损伤相关，乙醇给药导致肝脏和血浆脂质过氧化均显著增加。维生素（C和E）处理减轻了肝脏和血浆脂质过氧化。
维生素C和E可预防肝损伤和功能障碍，减轻脂质过氧化，因此在肝再生过程中，它们对乙醇介导的毒性作用似乎比维生素A显著更有效。
