Vosges Mélanie, Braguer Jean-Claude, Combarnous Yves
INRA-CNRS-University of Tours, Physiologie de la Reproduction et des Comportements, 37380 Nouzilly, France.
Reprod Toxicol. 2008 Feb;25(2):161-8. doi: 10.1016/j.reprotox.2007.12.002. Epub 2007 Dec 23.
Male rats were exposed to 17alpha-ethinylestradiol (EE2) in their drinking water either at concentrations of 1-1000 ng/mL (1 ppb to 1 ppm) for 3 weeks immediately after weaning from post-natal day 22 (PND 22) to PND 43 or at a concentration of 10 ng/mL from PND 5 onwards. Although given temporarily between PND 22 and PND 43, the 0.1 and 1 ppm doses of EE2 were found to significantly affect the ponderal growth of the animals. No male was found to be sterile neither in both exposed groups nor in control groups. Nevertheless, the males exposed to 10 ng/mL EE2 in drinking water from PND 5 onwards conceived a significantly higher proportion (25%) of small litters (one to five pups) than control males (0-3%). When exposed to 1 microg/mL EE2 but only from PND 22 to PND 43, the males produced an intermediate proportion (15%) of small litters in their progeny but this might be consecutive to the observed retardation in weight gain.
雄性大鼠在出生后第22天（PND 22）断奶至PND 43后的3周内，饮用含有浓度为1 - 1000纳克/毫升（1 ppb至1 ppm）的17α-乙炔雌二醇（EE2）的水，或者从PND 5开始饮用浓度为10纳克/毫升的EE2。尽管在PND 22至PND 43期间是短期给予，但发现0.1 ppm和1 ppm剂量的EE2会显著影响动物的体重增长。在暴露组和对照组中均未发现雄性不育。然而，从PND 5开始饮用含10纳克/毫升EE2的水的雄性大鼠，其产仔数少（一至五只幼崽）的比例（25%）显著高于对照雄性大鼠（0 - 3%）。当仅在PND 22至PND 43期间暴露于1微克/毫升的EE2时，雄性大鼠后代中产仔数少的比例为中等水平（15%），但这可能与观察到的体重增加迟缓有关。
