通过针对高氯酸盐还原酶基因的实时聚合酶链反应对高氯酸盐还原菌进行定量检测。
Quantitative detection of perchlorate-reducing bacteria by real-time PCR targeting the perchlorate reductase gene.
作者信息
Nozawa-Inoue Mamie, Jien Mercy, Hamilton Nicholas S, Stewart Valley, Scow Kate M, Hristova Krassimira R
机构信息
Department of Land, Air, and Water Resources, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA.
出版信息
Appl Environ Microbiol. 2008 Mar;74(6):1941-4. doi: 10.1128/AEM.01658-07. Epub 2008 Feb 1.
Abstract
A quantitative real-time PCR assay targeting the pcrA gene, encoding the catalytic subunit of perchlorate reductase, detected pcrA genes from perchlorate-reducing bacteria in three different genera and from soil microbial communities. Partial pcrA sequences indicated differences in the composition of perchlorate-reducing bacterial communities following exposure to different electron donors.
摘要
一种针对编码高氯酸盐还原酶催化亚基的pcrA基因的定量实时PCR检测方法，检测到了来自三个不同属的高氯酸盐还原细菌以及土壤微生物群落中的pcrA基因。部分pcrA序列表明，在接触不同电子供体后，高氯酸盐还原细菌群落的组成存在差异。
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3
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Appl Environ Microbiol. 2005 Jul;71(7):3928-34. doi: 10.1128/AEM.71.7.3928-3934.2005.
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Isolation and characterization of autotrophic, hydrogen-utilizing, perchlorate-reducing bacteria.
Appl Microbiol Biotechnol. 2005 Apr;67(2):261-8. doi: 10.1007/s00253-004-1725-0. Epub 2004 Sep 4.
5
Tracking nitrate reducers and denitrifiers in the environment.
Biochem Soc Trans. 2005 Feb;33(Pt 1):200-4. doi: 10.1042/BST0330200.
6
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Environ Microbiol. 2004 May;6(5):517-27. doi: 10.1111/j.1462-2920.2004.00598.x.
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