Radwan Samir S, Dashti Narjes, El-Nemr Israa, Khanafer Majida
Department of Biological Sciences, Faculty of Science, Kuwait University, Safat, Kuwait.
Int J Phytoremediation. 2007 Nov-Dec;9(6):475-86. doi: 10.1080/15226510701709580.
Standard and locally isolated nodule bacteria and plant growth-promoting rhizobacteria (PGPR) were grown on crude oil and individual pure hydrocarbons as sole sources of carbon and energy. The nodule bacteria included two standard Rhizobium leguminosarum strains, two standard Bradyrhizobium japonicum strains, and one unknown nodule bacterial strain that was locally isolated from Vicia faba nodules. The PGPR included one standard Serratia liquefaciens strain and two locally isolated strains of Pseudomonas aeruginosa and Flavobacterium sp. The pure hydrocarbons tested included n-alkanes with chain lengths from C9 to C40 and the aromatic hydrocarbons benzene, biphenyle, naphthalene, phenanthrene, and toluene. Quantitative gas liquid chromatographic analyses confirmed that pure cultures of representative nodule bacteria and PGPR could attenuate n-octadecane and phenanthrene in the surrounding nutrient medium. Further, intact nodules of V. faba containing bacteria immobilized on and within those nodules reduced hydrocarbon levels in a medium in which those nodules were shaken. It was concluded that legume crops are suitable phytoremediation tools for oily soil, since they enrich such soils not only with fixed nitrogen, but also with hydrocarbon-utilizing microorganisms. Further, legume nodules may have biotechnological value as materials for cleaning oily liquid wastes.
标准的以及从当地分离出的根瘤菌和促植物生长根际细菌（PGPR）以原油和单一的纯碳氢化合物作为唯一的碳源和能源进行培养。根瘤菌包括两株标准的豌豆根瘤菌菌株、两株标准的大豆慢生根瘤菌菌株，以及从蚕豆根瘤中分离出的一株未知根瘤菌菌株。促植物生长根际细菌包括一株标准的液化沙雷氏菌菌株，以及从当地分离出的两株铜绿假单胞菌和黄杆菌菌株。所测试的纯碳氢化合物包括链长从C9到C40的正构烷烃以及芳香烃苯、联苯、萘、菲和甲苯。定量气相色谱分析证实，代表性根瘤菌和促植物生长根际细菌的纯培养物能够降低周围营养培养基中正十八烷和菲的含量。此外，含有固定在根瘤上及根瘤内细菌的完整蚕豆根瘤，在其被振荡的培养基中可降低碳氢化合物水平。得出的结论是，豆科作物是油性土壤的合适植物修复工具，因为它们不仅能为这类土壤富集固定氮，还能富集利用碳氢化合物的微生物。此外，豆科植物根瘤作为清洁含油液体废物的材料可能具有生物技术价值。
