Gerbi Marleny E Martinez, Marques Aparecida M C, Ramalho Luciana Maria Pedreira, Ponzi Elizabeth Arruda Carneiro, Carvalho Carolina Montagn, Santos Rafael De Carneiro, Oliveira Priscila Chagas, Nóia Manuela, Pinheiro Antonio Luiz B
School of Dentistry, Federal University of Pernambuco, Recife, PE, Salvador.
Photomed Laser Surg. 2008 Feb;26(1):55-60. doi: 10.1089/pho.2007.2026.
This study assessed histologically the effect of laser photobiomodulation (LPBM) on the repair of surgical defects created in the femurs of Wistar rats treated or not treated with bone morphogenic proteins (BMPs) and organic bovine bone graft.
This paper is part of an ongoing series of works in which biomaterials are used in association with LPBM. Several previous reports by our group have shown that the use of laser photobiomodulation improves the treatment of bone defects.
Forty-eight adult male Wistar rats were divided into four randomized groups: group I (control, n = 12); group II (LPBM, n = 12); group III (BMPs + organic bovine bone graft, n = 12); and group IV (BMPs + organic bovine bone graft + LPBM, n = 12). The irradiated groups received seven irradiations every 48 h, beginning immediately after the surgical procedure. The laser therapy (lambda = 830 nm, 40 mW CW, varphi = 0.6 mm) consisted of 16 J/cm(2) per session divided equally over four points (4 J/cm(2) each) around the defect. The subjects were sacrificed after 15, 21, and 30 d, and the specimens were routinely embedded in wax, stained with hematoxylin and eosin and sirius red, and analyzed under light microscopy.
The results showed histological evidence of increased deposition of collagen fibers (at 15 and 21 d), as well as an increased amount of well-organized bone trabeculae at the end of the experimental period (30 d) in the irradiated animals versus the non-irradiated controls.
The use of LPBM with BMPs and organic bovine bone grafts increases the positive biomodulating effects of laser light.
本研究通过组织学方法评估了激光光生物调节（LPBM）对经骨形态发生蛋白（BMPs）和有机牛骨移植治疗或未治疗的Wistar大鼠股骨手术缺损修复的影响。
本文是正在进行的一系列研究的一部分，其中生物材料与LPBM联合使用。我们小组之前的几份报告表明，激光光生物调节的使用改善了骨缺损的治疗。
48只成年雄性Wistar大鼠被随机分为四组：第一组（对照组，n = 12）；第二组（LPBM组，n = 12）；第三组（BMPs + 有机牛骨移植组，n = 12）；第四组（BMPs + 有机牛骨移植 + LPBM组，n = 12）。照射组在手术后立即开始，每48小时接受7次照射。激光治疗（波长 = 830 nm，连续波40 mW，光斑直径 = 0.6 mm）每次照射剂量为16 J/cm²，在缺损周围四个点平均分配（每个点4 J/cm²）。在15、21和30天后处死动物，标本常规石蜡包埋，苏木精-伊红和天狼星红染色，在光学显微镜下分析。
结果显示，与未照射的对照组相比，照射组动物在实验期结束时（30天）组织学证据表明胶原纤维沉积增加（在15和21天），以及排列良好的骨小梁数量增加。
LPBM与BMPs和有机牛骨移植联合使用可增强激光的正向生物调节作用。
