Márquez Martínez Marleny Elizabeth, Pinheiro Antonio Luiz Barbosa, Ramalho Luciana Maria Pedreira
School of Dentistry, Pernambuco University, Camaragibe, Pernambuco, Brazil.
Lasers Med Sci. 2008 Jul;23(3):313-7. doi: 10.1007/s10103-007-0488-0. Epub 2007 Sep 20.
A major problem on modern dentistry is the recovery of bone defects of different etiologies. Biomaterials are used to improve the repair of these defects. Previous studies have shown positive effects of Laser Photobiomodulation (LPBM) on the repair of both soft and bone tissues. This study assessed histologically the effect of LPBM on the repair of surgical defects on the femur of rats filled with lyophilized bovine bone. The animals were divided into three groups: group I (control); group II (graft); group III (graft + LPBM). The animals on the irradiated groups received 16 J/cm(2) per session divided into four points around the defect being the first irradiation immediately after surgery and repeated at every 48 h during 2 weeks. Animal death occurred 15, 21, and 30 days after surgery. The specimens were routinely processed and stained with H/E and Sirius red and analyzed by light microscopy. There was histological evidence of improved collagen fiber deposition at early stages of the healing; increased amount of well-organized bone trabeculae at the end of the experimental period on irradiated animals. It is concluded that LPBM has positive biomodulative effect on the healing process bone defects.
现代牙科的一个主要问题是不同病因导致的骨缺损的修复。生物材料被用于改善这些缺损的修复。先前的研究表明，激光光生物调节（LPBM）对软组织和骨组织的修复均有积极作用。本研究通过组织学方法评估了LPBM对填充冻干牛骨的大鼠股骨手术缺损修复的影响。将动物分为三组：第一组（对照组）；第二组（移植组）；第三组（移植 + LPBM组）。照射组的动物每次接受16 J/cm²，分为缺损周围的四个点，首次照射在手术后立即进行，并在2周内每48小时重复一次。动物在手术后15、21和30天死亡。标本常规处理后用苏木精/伊红（H/E）和天狼星红染色，并通过光学显微镜进行分析。有组织学证据表明，在愈合早期胶原纤维沉积有所改善；在实验期结束时，照射组动物排列良好的骨小梁数量增加。得出的结论是，LPBM对骨缺损的愈合过程具有积极的生物调节作用。
