Houhamdi Linda, Raoult Didier
Unité des Rickettsies, Faculté de Médecine, Institut Fédératif de Recherche 48, Centre National de Recherche Scientifique, Unité Mixte de Recherche 6020, Université de la Méditerranée, 27, Boulevard Jean Moulin, 13385 Marseille Cedex 5, France.
Microb Pathog. 2008 May;44(5):435-7. doi: 10.1016/j.micpath.2007.11.007. Epub 2007 Dec 14.
To assess the role of virulence factors identified in Caenorhabditis elegans in the transmission of plague by lice, we infected 100 lice by feeding them on rabbits and made them bacteremic; the rabbits had been intravenously inoculated with 10(9) CFU of six different mutant Yersinia pestis strains of lower pathogenicity for C. elegans, obtained from the KIM5 strain. This strain lacks genes used for biofilm formation. High mortality rates were observed in all lice, which excreted viable bacteria in their feces. Mutants killed rabbits when infected intravenously, but mutants were not transmitted to rabbits by infected lice. We conclude that the genes governing pathogenicity in C. elegans and louse are not identical.
为评估在秀丽隐杆线虫中鉴定出的毒力因子在跳蚤传播鼠疫中的作用，我们通过让100只跳蚤以感染了细菌血症的兔子为食来感染它们；这些兔子已通过静脉注射从KIM5菌株获得的六种对秀丽隐杆线虫致病性较低的不同突变型鼠疫耶尔森菌菌株（10⁹CFU）。该菌株缺乏用于生物膜形成的基因。在所有跳蚤中均观察到高死亡率，它们在粪便中排出了活细菌。突变体通过静脉感染时会杀死兔子，但感染的跳蚤不会将突变体传播给兔子。我们得出结论，控制秀丽隐杆线虫和跳蚤致病性的基因并不相同。
