Han Jae Yong
Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of Korea.
Comp Immunol Microbiol Infect Dis. 2009 Mar;32(2):61-80. doi: 10.1016/j.cimid.2007.11.010. Epub 2008 Feb 4.
Chickens have proven to be useful organisms for transgenic research. This work provides enormous benefits in advancing animal biotechnology and aids in the development of unique technologies for bioreactor production and experimental model development. The various advantages of chicken transgenesis are derived from the genetic and physiological characteristics of this organism, although several physiological properties have impeded the development of an efficient transgenic system. We have developed embryo-mediated and testis-mediated transgenic systems using chicken primordial germ cells (PGCs) from embryos and testicular cells from adult males. These methods are efficient and involve minimal technical effort. Here, we review previous transgenic research using PGCs and testicular cells from chickens. Furthermore, we have summarized the development of the chicken model system in biomedical science and biotechnology and our recent achievements in this field.
鸡已被证明是转基因研究中有用的生物体。这项工作在推进动物生物技术方面带来了巨大益处，并有助于开发用于生物反应器生产和实验模型开发的独特技术。鸡转基因的各种优势源于该生物体的遗传和生理特征，尽管一些生理特性阻碍了高效转基因系统的开发。我们利用来自胚胎的鸡原始生殖细胞（PGCs）和成年雄性的睾丸细胞开发了胚胎介导和睾丸介导的转基因系统。这些方法效率高且技术难度小。在此，我们回顾了以前使用鸡的PGCs和睾丸细胞进行的转基因研究。此外，我们总结了鸡模型系统在生物医学科学和生物技术中的发展以及我们在该领域的最新成果。
