Tuderman L
Eur J Biochem. 1976 Jul 15;66(3):615-21. doi: 10.1111/j.1432-1033.1976.tb10589.x.
Changes in prolyl hydroxylase activity and immunoreactive protein were studied in various chick embryo tissues during the embryonic development. Both the enzyme activity and the amoung of immunoreactive protein increased till the 16th day of development and declined thereafter in all tissues studied. Comparison of the enzyme activity to the content of the total immuno-reactive protein indicated that there are distinct differences in the degree of enzyme activity between different chick embryo tissues, and in the same tissue between different stages of embryonic development. The highest relative enzyme activities were found in cartilage and skin, in which about 60% of the enzyme was active on the 16th day of development and only 20-30% was active on the 20th day of development; the lowest values were observed in spleen and large vessels, in which below 10% of the enzyme protein was in the active form on the 20th day of development Gel filtration studies demonstrated that in cartilage of 16-day-old chick embryos about 60% of the total immunoreactive enzyme in the tissue was present in the form of active prolylhydroxylase tetramer, whereas on the 20th day of development only 30% of the enzyme protein in cartilage was in the tetramer form. By contrast, in large vessels of the 16-day-old chick embryos, essentially all the enzyme was in the form of prolyl hydroxylase monomers.
在胚胎发育过程中，对不同鸡胚组织中脯氨酰羟化酶活性和免疫反应性蛋白的变化进行了研究。在所研究的所有组织中，酶活性和免疫反应性蛋白的量在发育的第16天之前均增加，此后下降。酶活性与总免疫反应性蛋白含量的比较表明，不同鸡胚组织之间以及同一组织在胚胎发育的不同阶段之间，酶活性程度存在明显差异。在软骨和皮肤中发现相对酶活性最高，其中在发育的第16天约60%的酶具有活性，而在发育的第20天只有20%-30%具有活性；在脾脏和大血管中观察到最低值，在发育的第20天，低于10%的酶蛋白呈活性形式。凝胶过滤研究表明，在16日龄鸡胚的软骨中，组织中总免疫反应性酶约60%以活性脯氨酰羟化酶四聚体的形式存在，而在发育的第20天，软骨中只有30%的酶蛋白呈四聚体形式。相比之下，在16日龄鸡胚的大血管中，基本上所有的酶都以脯氨酰羟化酶单体的形式存在。
