Berti E, Aversa G G, Soligo D, Cattoretti G, Delia D, Aiello A, Parravicini C, Hall B M, Caputo R
Department of Clinica Dermatologica I, University of Milano, Italy.
Am J Clin Pathol. 1991 Feb;95(2):188-93. doi: 10.1093/ajcp/95.2.188.
The authors report on the extensive characterization, on normal and pathologic tissues, of the T-cell-specific monoclonal antibody (MoAb) A6, which the authors previously found to identify a fixation- and paraffin-embedding-resistant epitope. A6 reacted with most T lymphocytes, macrophages, and Langerhans' cells of normal tissues and with peripheral T-cell lymphomas (31 of 34), Ki-1+ lymphomas (12 of 18), and T-cell leukemias (1 of 5). All cases of X and non-X histiocytosis examined and monocytic leukemias with mature phenotype only were A6 positive. Three of 47 cases of B-cell lymphoma and leukemia were labeled. Hairy cell leukemias, multiple myelomas, and Hodgkin's and Reed-Sternberg cells were negative. The A6 reactivity was preserved with different fixatives (formalin, Bouin's fluid, Carnoy's fixative, and B5) and decalcification procedures and was slightly enhanced by trypsin digestion. The pattern of reactivity of A6 was similar to that obtained with MoAb UCHL-1, recognizing the CD45RO determinant of leukocyte common antigen; however, in pathologic tissues, A6 labeled a higher percentage of cells than UCHL-1. Cross-blocking and enzyme digestion studies (Pronase E [Sigma Chemical, St. Louis, MO] and neuraminidase [Sigma Chemical]) indicated that the two MoAbs may identify close epitopes on the same molecule. In conclusion, the authors' study indicates that A6 is an excellent reagent for detection of the CD45RO molecule on paraffin-embedded normal and pathologic tissues.
作者报告了对T细胞特异性单克隆抗体（MoAb）A6在正常和病理组织上的广泛特性分析，作者之前发现该抗体可识别一种抗固定和石蜡包埋的表位。A6与正常组织中的大多数T淋巴细胞、巨噬细胞和朗格汉斯细胞反应，也与外周T细胞淋巴瘤（34例中的31例）、Ki-1 +淋巴瘤（18例中的12例）和T细胞白血病（5例中的1例）反应。所有检查的X和非X组织细胞增多症病例以及仅具有成熟表型的单核细胞白血病均为A6阳性。47例B细胞淋巴瘤和白血病中有3例被标记。毛细胞白血病、多发性骨髓瘤以及霍奇金和里德-斯特恩伯格细胞均为阴性。A6与不同固定剂（福尔马林、波因氏液、卡诺氏固定剂和B5）以及脱钙程序反应后仍保持活性，经胰蛋白酶消化后活性略有增强。A6的反应模式与识别白细胞共同抗原CD45RO决定簇的MoAb UCHL-1相似；然而，在病理组织中，A6标记的细胞百分比高于UCHL-1。交叉阻断和酶消化研究（链霉蛋白酶E [西格玛化学公司，密苏里州圣路易斯]和神经氨酸酶 [西格玛化学公司]）表明，这两种单克隆抗体可能识别同一分子上的紧密表位。总之，作者的研究表明，A6是检测石蜡包埋的正常和病理组织上CD45RO分子的优良试剂。
