Kawaguchi H, Miyashita T, Herbst H, Niedobitek G, Asada M, Tsuchida M, Hanada R, Kinoshita A, Sakurai M, Kobayashi N
Department of Virology, National Children's Medical Research Center, Tokyo, Japan.
J Clin Invest. 1993 Sep;92(3):1444-50. doi: 10.1172/JCI116721.
The clonal composition of EBV-infected cells was examined in three cases of EBV-associated hemophagocytic syndrome by analysis of the heterogeneity of terminal repetitive sequences in the EBV genome, indicating monoclonal expansion of EBV-infected cells in all cases. Involvement of T lymphoid cells was determined by in situ hybridization using 35S-labeled RNA probes specific for the small EBV-encoded nuclear RNAs, EBER1 and EBER2, in combination with immunostaining for the TCR-beta chain, CD45RO, CD20, CD30 and CD68 antigens in these three cases. The majority of lymphoid cells showing EBER transcripts were stained by antibodies against CD45RO and T cell receptor-beta. In contrast, EBER-specific signals were not detectable on B cells or hemophagocytic cells. These data support the concept that EBV-associated T cell proliferation is a primary feature of EBV-AHS.
通过分析EBV基因组中末端重复序列的异质性，对3例EBV相关噬血细胞综合征病例中EBV感染细胞的克隆组成进行了检测，结果表明所有病例中EBV感染细胞均呈单克隆扩增。采用针对小EBV编码核RNA EBER1和EBER2的35S标记RNA探针进行原位杂交，并结合对这3例病例中TCR-β链、CD45RO、CD20、CD30和CD68抗原的免疫染色，确定T淋巴细胞的受累情况。大多数显示EBER转录本的淋巴细胞被抗CD45RO和T细胞受体-β抗体染色。相反，在B细胞或噬血细胞上未检测到EBER特异性信号。这些数据支持了EBV相关T细胞增殖是EBV-AHS主要特征这一概念。
