抗氧化剂DL-α硫辛酸对阿霉素诱导大鼠模型肝毒性的减轻作用

Antioxidant DL-alpha lipoic acid as an attenuator of adriamycin induced hepatotoxicity in rat model.

作者信息

Anandakumar Padmavathi Pandi, Malarkodi Saroja Palanichamy, Sivaprasad Thylambal Rajagopalan, Saravanan Gandhimathi Dhravidamani

机构信息

Department of Biochemistry, University of Seychelles-American Institute of Medicine, International Study Centre, Administrated by Aashirwad Health and Education Trust, Mavuthampathy Post, Navakarai, Coimbatore 641 105, India.

出版信息

Indian J Exp Biol. 2007 Dec;45(12):1045-9.

PMID:18254210

Abstract

Protective efficacy of DL-alpha lipoic acid on adriamycin induced hepatotoxicity was evaluated in rats. Adriamycin toxicity, induced by a single injection (ip; 15 mg/kg body wt), was expressed by an elevation in alanine transaminase, aspartate transaminase, bilirubin levels in serum and alkaline phosphatase, lactate dehydrogenase, alanine transaminase, aspartate transaminase activity in hepatic tissue. Adriamycin produced significant increase in malondialdehyde levels indicating tissue lipid peroxidation and potentially inhibiting the activity of antioxidant, reduced glutathione and antioxidant enzymes, catalase, superoxide dismutase, glutathione peroxidase, glutathione reductase, glutathione-S-transferase, glucose-6-phosphate dehydrogenase. The present results showed that pretreatment with lipoic acid [75 mg/kg body wt/day (ip), 24 h prior to administration of adriamycin] significantly restored various cellular activity suggesting the antioxidant potential of lipoic acid in ameliorating the hepatotoxicity induced by adriamycin.

摘要

在大鼠中评估了DL-α硫辛酸对阿霉素诱导的肝毒性的保护作用。单次注射（腹腔注射；15毫克/千克体重）诱导的阿霉素毒性表现为血清中丙氨酸转氨酶、天冬氨酸转氨酶、胆红素水平以及肝组织中碱性磷酸酶、乳酸脱氢酶、丙氨酸转氨酶、天冬氨酸转氨酶活性升高。阿霉素使丙二醛水平显著升高，表明组织脂质过氧化，并可能抑制抗氧化剂、还原型谷胱甘肽以及抗氧化酶过氧化氢酶、超氧化物歧化酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶、谷胱甘肽-S-转移酶、葡萄糖-6-磷酸脱氢酶的活性。目前的结果表明，硫辛酸预处理[75毫克/千克体重/天（腹腔注射），在给予阿霉素前24小时]显著恢复了各种细胞活性，表明硫辛酸在改善阿霉素诱导的肝毒性方面具有抗氧化潜力。