Bañuls María-José, García-Piñón Francisco, Puchades Rosa, Maquieira Angel
Instituto de Química Molecular Aplicada, Departamento de Química, Universidad Politécnica de Valencia, Camino de Vera s/n,Valencia, Spain.
Bioconjug Chem. 2008 Mar;19(3):665-72. doi: 10.1021/bc7003457. Epub 2008 Feb 7.
Compact discs have been proposed as an efficient analytical platform, with potential to develop high-throughput affinity assays for genomics, proteomics, clinics, and health monitoring. Chemical derivatization of CD surfaces is one of the keys to developing highly efficient microarraying-based assays on discs. Approaches for mild chemical modification of polycarbonate (PC) disc surface based on nitration, reduction, and chloromethylation reactions have been developed. Derivatized surfaces as amino and thiol are obtained for PC, maintaining unchanged the mechanical and optical properties of the discs. Studies of covalent attachment of oligonucleotide probes (5' Cy5-labeled, 3' NH 2-ended) on the modified surfaces have been performed to develop microarraying assays based on hybridization of cDNA strands and single nucleotide polymorphism discrimination (SNPs). A demonstration of the applicability to the compact disc audio/video technology for its use as analytical system is performed, including the employment of a commercial CD player to read the results on disc.
光盘已被提议作为一种高效的分析平台，有潜力开发用于基因组学、蛋白质组学、临床和健康监测的高通量亲和分析方法。光盘表面的化学衍生化是在光盘上开发基于高效微阵列分析方法的关键之一。基于硝化、还原和氯甲基化反应，已开发出对聚碳酸酯（PC）光盘表面进行温和化学修饰的方法。通过这些方法可使PC光盘表面衍生出氨基和硫醇基，同时保持光盘的机械和光学性能不变。已开展了在修饰表面上共价连接寡核苷酸探针（5'端用Cy5标记，3'端为NH₂末端）的研究，以开发基于cDNA链杂交和单核苷酸多态性鉴别（SNP）的微阵列分析方法。还展示了该光盘音频/视频技术作为分析系统的适用性，包括使用商用CD播放器读取光盘上的结果。
