Departamento de Química, Instituto de Reconocimiento Molecular y Desarrollo Tecnológico, Universidad Politécnica de Valencia, Camino de Vera s/n, 46071 Valencia, Spain.
Biosens Bioelectron. 2011 Jan 15;26(5):2694-8. doi: 10.1016/j.bios.2010.08.066. Epub 2010 Sep 6.
Direct hapten-linked multiplexed immunoassay is developed on the polycarbonate surface of standard Digital Versatile Discs (DVDs) for six compounds of environmental concern, as proof of concept. Carboxylated haptens are directly linked to the aminated polycarbonate surface through carbodiimide/succinimide coupling. The modified DVD surface maintained its physical and optical properties. Multiplexed assay reached detection limits down to 0.1 μg/L for chlorpyrifos, 2,4,5-trichlorophenoxypropionic acid, sulfathiazole and sulfasalazine and down to 1.0 μg/L for fenthion and malathion. This approach presents advantages such as the improvement in sensitivity in comparison to protein-hapten conjugate format for all the studied analytes and the absence of cross-interference effects, allowing high throughput multianalysis on the same surface. Also, a comparison of the performance of two sensing strategies indicated that DVD disc and drive approach turned out in a simpler mode, the assays being more reproducible and with higher signal to noise ratios.
直接连接半抗原的多元免疫分析方法在标准数字多功能光盘（DVD）的聚碳酸酯表面上得到了发展，用于检测六种环境关注化合物，以此作为概念验证。羧基化半抗原通过碳二亚胺/琥珀酰亚胺偶联直接连接到氨基化聚碳酸酯表面。修饰后的 DVD 表面保持了其物理和光学性质。多元分析方法的检测限低至 0.1μg/L，可检测到氯吡硫磷、2,4,5-三氯苯氧丙酸、磺胺噻唑和柳氮磺胺吡啶，检测限低至 1.0μg/L，可检测到芬硫磷和马拉硫磷。与所有研究的分析物相比，这种方法在蛋白质-半抗原缀合物形式下提高了灵敏度，并且不存在交叉干扰效应，允许在同一表面上进行高通量多分析，具有优势。此外，两种传感策略的性能比较表明，DVD 光盘和驱动器方法更为简单，分析更具重现性，信噪比更高。
