Bañuls María-José, Gonzalez-Pedro Victoria, Puchades Rosa, Maquieira Angel
Instituto de Química Molecular Aplicada, Departamento de Química, Universidad Politécnica de Valencia, Camino de Vera s/n, Valencia, Spain.
Bioconjug Chem. 2007 Sep-Oct;18(5):1408-14. doi: 10.1021/bc700194n.
A mild chemical procedure for the derivatization of PMMA as the isocyanate on rigid supports is described. The proposal is based on spin-coating the support with a hydroxy-modified PMMA polymer, followed by treatment with (3-isocyanatopropyl)triethoxysilane, developing an isocyanate-ended PMMA. Oligonucleotide hybridization assays performed on this surface demonstrate that the process is simple and highly effective and agree with the results of other modified materials, including aminated PMMA, gold, and glass. As a demonstration, it has been applied to covalently attached aminated oligonucleotides on a modified audio-video compact disc (CD) surface to perform DNA probe hybridization assays. Measurements were carried out with a CD player, by detecting the solid microspots precipitated from an enzymatic reaction (HRP-TMB). This new approach, being a competitive technique, opens a broad horizon of applications for point of care or in situ needs.
描述了一种在刚性载体上对聚甲基丙烯酸甲酯（PMMA）进行衍生化以形成异氰酸酯的温和化学方法。该方法基于用羟基改性的PMMA聚合物旋涂载体，然后用（3-异氰酸酯基丙基）三乙氧基硅烷处理，形成异氰酸酯端基的PMMA。在该表面上进行的寡核苷酸杂交试验表明，该过程简单且高效，并且与其他改性材料（包括胺化PMMA、金和玻璃）的结果一致。作为示例，它已应用于在改性的音频视频光盘（CD）表面共价连接胺化寡核苷酸，以进行DNA探针杂交试验。通过检测酶促反应（HRP-TMB）沉淀的固体微斑点，用CD播放器进行测量。这种新方法作为一种有竞争力的技术，为即时护理或原位需求开辟了广阔的应用前景。
