通过带有杂芳族核碱基的2'，4'-BNA形成平行基序三链体来识别T.A中断。

Recognition of T.A interruption by 2',4'-BNAs bearing heteroaromatic nucleobases through parallel motif triplex formation.

作者信息

Obika Satoshi, Inohara Hiroyasu, Hari Yoshiyuki, Imanishi Takeshi

机构信息

Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.

出版信息

Bioorg Med Chem. 2008 Mar 15;16(6):2945-54. doi: 10.1016/j.bmc.2007.12.043. Epub 2007 Dec 25.

DOI:10.1016/j.bmc.2007.12.043

PMID:18255299

Abstract

2'-O,4'-C-methylene bridged nucleic acid (2',4'-BNA) monomers bearing novel unnatural nucleobases, 4-(3-benzamidophenyl)-2-pyridone and 2-(N-methylbenzamido)thiazole, were synthesized and successfully incorporated into oligonucleotides. UV melting experiments showed that the corresponding oligonucleotide derivatives formed stable triplexes with dsDNA targets even in the presence of a T.A interruption.

摘要

携带新型非天然核碱基4-(3-苯甲酰胺基苯基)-2-吡啶酮和2-(N-甲基苯甲酰胺基)噻唑的2'-O,4'-C-亚甲基桥连核酸(2',4'-BNA)单体被合成并成功掺入寡核苷酸中。紫外熔解实验表明，即使存在T·A中断，相应的寡核苷酸衍生物仍能与双链DNA靶标形成稳定的三链体。