G1/S 细胞周期蛋白依赖性激酶通过磷酸化酿酒酵母中的 RhoGEF Tus1p 来调节小 GTP 酶 Rho1p。
G1/S cyclin-dependent kinase regulates small GTPase Rho1p through phosphorylation of RhoGEF Tus1p in Saccharomyces cerevisiae.
作者信息
Kono Keiko, Nogami Satoru, Abe Mitsuhiro, Nishizawa Masafumi, Morishita Shinichi, Pellman David, Ohya Yoshikazu
机构信息
Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Chiba 277-8562, Japan.
出版信息
Mol Biol Cell. 2008 Apr;19(4):1763-71. doi: 10.1091/mbc.e07-09-0950. Epub 2008 Feb 6.
Abstract
Rho1p is an essential small GTPase that plays a key role in the morphogenesis of Saccharomyces cerevisiae. We show here that the activation of Rho1p is regulated by a cyclin-dependent kinase (CDK). Rho1p is activated at the G1/S transition at the incipient-bud sites by the Cln2p (G1 cyclin) and Cdc28p (CDK) complex, in a process mediated by Tus1p, a guanine nucleotide exchange factor for Rho1p. Tus1p interacts physically with Cln2p/Cdc28p and is phosphorylated in a Cln2p/Cdc28p-dependent manner. CDK phosphorylation consensus sites in Tus1p are required for both Cln2p-dependent activation of Rho1p and polarized organization of the actin cytoskeleton. We propose that Cln2p/Cdc28p-dependent phosphorylation of Tus1p is required for appropriate temporal and spatial activation of Rho1p at the G1/S transition.
摘要
Rho1p是一种必需的小GTP酶，在酿酒酵母的形态发生中起关键作用。我们在此表明，Rho1p的激活受细胞周期蛋白依赖性激酶（CDK）调控。在起始芽位点，Rho1p在G1/S转换时被Cln2p（G1期细胞周期蛋白）和Cdc28p（CDK）复合物激活，此过程由Tus1p介导，Tus1p是Rho1p的鸟嘌呤核苷酸交换因子。Tus1p与Cln2p/Cdc28p发生物理相互作用，并以Cln2p/Cdc28p依赖的方式被磷酸化。Tus1p中的CDK磷酸化共有序列位点对于Rho1p的Cln2p依赖性激活和肌动蛋白细胞骨架的极化组织都是必需的。我们提出，Tus1p的Cln2p/Cdc28p依赖性磷酸化对于Rho1p在G1/S转换时的适当时间和空间激活是必需的。
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EMBO J. 2007 Oct 31;26(21):4487-500. doi: 10.1038/sj.emboj.7601847. Epub 2007 Sep 13.
2
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3
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4
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5
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6
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7
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