Gray D A, Schütz H, Gerstberger R
Department of Physiology I, Max-Planck Institute, W. G. Kerckhoff Institute for Physiological and Clinical Research, Bad Nauheim, Germany.
Endocrinology. 1991 Mar;128(3):1655-60. doi: 10.1210/endo-128-3-1655.
A RIA was developed for the measurement of immunoreactive atrial natriuretic factor (irANF) in avian plasma and was used to investigate the relationship between vascular volume and plasma irANF concentrations in conscious Pekin ducks. In normally hydrated birds, the mean plasma irANF concentration was 78.5 +/- 6.8 pg/ml. Blood volume expansion by iv infusion of isotonic saline at 1 ml/min elevated irANF concentrations by 132% after 1 h and by 233% after 2 h. Reduction of the vascular volume by nonhypotensive hemorrhage of 10% and then 20% of the total blood volume reduced irANF levels by 16% and 42%, respectively, 5 min after blood removal was completed. Similarly, in birds deprived of water for 24 h and with blood volume reduced by 4.8%, plasma irANF concentrations were 50% lower than in the same euhydrated animals. The observed correlation between plasma irANF concentrations and vascular volume is consistent with the concept that ANF has a physiological role in avian volume homeostasis.
我们开发了一种放射免疫分析方法（RIA），用于测量禽类血浆中免疫反应性心房钠尿肽（irANF），并利用该方法研究了清醒北京鸭血管容量与血浆irANF浓度之间的关系。在正常水合状态的鸟类中，血浆irANF的平均浓度为78.5±6.8 pg/ml。以1 ml/min的速度静脉输注等渗盐水使血容量增加，1小时后irANF浓度升高了132%，2小时后升高了233%。通过非低血压性出血分别减少总血容量的10%和20%来降低血管容量，放血结束5分钟后，irANF水平分别降低了16%和42%。同样，在缺水24小时且血容量减少4.8%的鸟类中，血浆irANF浓度比相同的正常水合动物低50%。观察到的血浆irANF浓度与血管容量之间的相关性与心房钠尿肽在禽类容量稳态中具有生理作用的概念一致。
