Mythilypriya R, Shanthi P, Sachdanandam P
Department of Medical Biochemistry, Dr. ALM. Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai, 600 113, India.
Inflammopharmacology. 2008 Feb;16(1):21-35. doi: 10.1007/s10787-007-1602-4.
The present study was performed in order to establish the efficacy of Kalpaamruthaa (KA), a modified indigenous Siddha preparation in adjuvant induced arthritic rat (AIA) model with reference to mediators of inflammation (lysosomal enzymes) and its effect on proteoglycans. Albino rats of Wistar strain were divided into seven Groups of six animals each. Arthritis was induced to rats by subcutaneous injection of 0.1 ml of Complete Freund's Adjuvant into the plantar surface of the left hind paw. Group I served as normal control rats receiving 0.5 ml of olive oil as vehicle, Group II arthritic rats served as induced-untreated and Group III (50 mg/kg), Group IV (100 mg/kg), Group V (150 mg/kg), Group VI (200 mg/kg) and Group VII (250 mg/kg) were KA treated rats at different dose levels orally in 0.5 ml of olive oil from 14(th) day of adjuvant injection and was terminated on day 28. Animals were then sacrificed on the day 29, blood was collected, liver and kidney were dissected out, washed and 10% homogenates were prepared. The activities of lysosomal enzymes (beta-glucuronidase, beta-galactosidase, acid phosphatase, beta-N-acetyl glucosaminidase and cathepsin-D), aminotransferases (alkaline phosphatase, aspartate and aminotransferases) and levels of plasma protein bound carbohydrate components of glycoproteins were determined and were found to be elevated in arthritic rats when compared to control animals. After administration of KA, the activities of lysosomal enzymes, aminotransferases and protein-bound carbohydrate component levels were significantly normalized. The data obtained evidently indicate that Kalpaamruthaa is effective at the dose of 150 mg/kg b.wt. in AIA and plays an important role in lysosomal membrane stabilization. This was further confirmed by radiological, histological and electron microscopic studies.
本研究旨在建立改良的印度传统悉达疗法制剂Kalpaamruthaa（KA）在佐剂诱导的关节炎大鼠（AIA）模型中的疗效，涉及炎症介质（溶酶体酶）及其对蛋白聚糖的影响。将Wistar品系的白化大鼠分为七组，每组六只动物。通过在左后爪足底皮下注射0.1 ml完全弗氏佐剂诱导大鼠患关节炎。第一组作为正常对照大鼠，接受0.5 ml橄榄油作为赋形剂；第二组为诱导未治疗的关节炎大鼠；第三组（50 mg/kg）、第四组（100 mg/kg）、第五组（150 mg/kg）、第六组（200 mg/kg）和第七组（250 mg/kg）为从佐剂注射第14天起以0.5 ml橄榄油口服不同剂量水平KA治疗的大鼠，并在第28天处死。然后在第29天处死动物，采集血液，取出肝脏和肾脏，冲洗后制备10%匀浆。测定溶酶体酶（β-葡萄糖醛酸酶、β-半乳糖苷酶、酸性磷酸酶、β-N-乙酰氨基葡萄糖苷酶和组织蛋白酶-D）、氨基转移酶（碱性磷酸酶、天冬氨酸和氨基转移酶）的活性以及糖蛋白中血浆蛋白结合碳水化合物成分的水平，发现与对照动物相比，关节炎大鼠的这些指标均升高。给予KA后，溶酶体酶、氨基转移酶的活性以及蛋白结合碳水化合物成分水平均显著恢复正常。所获得的数据明显表明，Kalpaamruthaa在AIA模型中150 mg/kg体重剂量下有效，并在溶酶体膜稳定中起重要作用。这通过放射学、组织学和电子显微镜研究得到进一步证实。
