Chen Yong, Wang Qi-Wen, Zuo Jian, Chen Jian-Wei, Li Xiang
College of Pharmacy, Nanjing University of Chinese Medicine, POB 210023, Nanjing, China.
Jiangsu Key Laboratory of Therapeutic Material of Chinese Medicine, POB 210023 , Nanjing, China.
BMC Complement Altern Med. 2017 Jan 5;17(1):11. doi: 10.1186/s12906-016-1500-7.
Claoxylon indicum Hassk. (Euphorbiaceae), named Diu Le Bang, have functions of dehumidification and relieving swelling pain, and is used as a folk medicine to treat Rheumatoid arthritis (RA), lumbocrural pain and foot edema in the south of China. The aim of the present study was to investigate the anti-arthritic activity of the ethanol extract of Claoxylon indicum (CIE) on mice with adjuvant induced joint arthritis.
Adjuvant arthritis was induced in mice by subcutaneous injection of complete Freund's adjuvant into the plantar surface of right hind paw. Arthritis severity was evaluated by arthritic score, hind paws oedema and spleen index, and histological examinations. Serum samples were collected for determination of malondialdehyde (MDA) and alkaline phosphatase (ALP) levels. The expression of interleukin-1 beta (IL-1β) and tumor necrosis factor alpha (TNF-α) in the specimens of knee joints was determined by standard immunohistochemical techniques.
CIE administration (0.4 and 0.8 g/kg) suppressed the inflammatory responses in the joints of adjuvant-induced arthritis (AIA) mice, suggested by the modulatory effects on paw swelling, hyperplasia of lymphatic tissues and synovial membrane. It also decreased the levels of MDA and ALP in serum and downregulated the expression of IL-1β and TNF-α in the arthritic joints of AIA mice.
These results suggested that CIE possessed substantial anti-arthritic activity due to immumodepression and regulation of cytokines. CIE may be a potential candidate for the treatment of RA.
白桐树（大戟科），名为丢勒棒，具有除湿和缓解肿痛的功效，在中国南方被用作民间药物治疗类风湿性关节炎（RA）、腰腿痛和足部水肿。本研究的目的是探讨白桐树乙醇提取物（CIE）对佐剂诱导性关节关节炎小鼠的抗关节炎活性。
通过将完全弗氏佐剂皮下注射到右后爪足底表面诱导小鼠患佐剂性关节炎。通过关节炎评分、后爪水肿和脾脏指数以及组织学检查来评估关节炎严重程度。收集血清样本以测定丙二醛（MDA）和碱性磷酸酶（ALP）水平。通过标准免疫组织化学技术测定膝关节标本中白细胞介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）的表达。
CIE给药（0.4和0.8 g/kg）抑制了佐剂诱导性关节炎（AIA）小鼠关节中的炎症反应，这通过对爪肿胀、淋巴组织和滑膜增生的调节作用得以体现。它还降低了血清中MDA和ALP的水平，并下调了AIA小鼠关节炎关节中IL-1β和TNF-α的表达。
这些结果表明，CIE由于免疫抑制和细胞因子调节而具有显著的抗关节炎活性。CIE可能是治疗RA的潜在候选药物。
