反相高效液相色谱法同时测定咸鸭蛋蛋黄中角黄素和苏丹红

[Simultaneous determination of canthaxanthin and Sudan Red in salted duck egg yolk by reversed-phase high performance liquid chromatography].

作者信息

Wang Quanlin, Shi Pingping, Zhang Shufen, Shen Jian, Fu Xiao

机构信息

Supervision and Inspection Institute of Product Quality, Ningbo 315041, China.

出版信息

Se Pu. 2007 Nov;25(6):864-6.

PMID:18257306

Abstract

A reversed-phase high performance liquid chromatographic (RP-HPLC) method for the simultaneous determination of canthaxanthin, Sudan Red I , Sudan Red II , Sudan Red III and Sudan Red IV in salted duck egg yolk has been developed. These canthaxanthin and Sudan Red dyes were extracted with a mixture of acetonitrile, methanol, and chloroform (1 : 0.5 : 0.5, v/v). The extract was dried by a rotary evaporator. The residue was transferred to a graduated tube and diluted to 10 mL with acetonitrile. The canthaxanthin and Sudan Red dyes were separated on a XDB-C18 column (250 mm x 4.6 mm), with acetonitrile-water (95:5, v/v) as mobile phase. They were then detected at 478 nm - 520 nm - 471 nm at different retention times by UV/Vis spectrophotometer. The recoveries of sudan I , sudan II, sudan II, sudan IV, and canthaxanthin were 97.34%, 89.56%, 90.98%, 93.63% and 95.15% respectively. The relative standard deviations (RSDs) were 4.7%, 4.3%, 5.1%, 4.9% and 3.1%, respectively. The method is simple, rapid and accurate.

摘要

建立了一种反相高效液相色谱（RP-HPLC）法，用于同时测定咸鸭蛋蛋黄中的角黄素、苏丹红I、苏丹红II、苏丹红III和苏丹红IV。这些角黄素和苏丹红染料用乙腈、甲醇和氯仿的混合物（1:0.5:0.5，v/v）萃取。提取物用旋转蒸发仪干燥。将残留物转移至刻度管中，并用乙腈稀释至10 mL。角黄素和苏丹红染料在XDB-C18柱（250 mm×4.6 mm）上分离，以乙腈-水（95:5，v/v）作为流动相。然后用紫外/可见分光光度计在不同保留时间于478 nm、520 nm、471 nm处进行检测。苏丹红I、苏丹红II、苏丹红III、苏丹红IV和角黄素的回收率分别为97.34%、89.56%、90.98%、93.63%和95.15%。相对标准偏差（RSD）分别为4.7%、4.3%、5.1%、4.9%和3.1%。该方法简单、快速、准确。