He Kang-Hao, Zou Xiao-Li, Liu Xiang, Zeng Hong-Yan
Department of Sanitary Technology, West China Public Health School, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2012 Jan;43(1):113-7.
A method using reversed phase high performance liquid chromatography (RP-HPLC) coupled with diode array detector (DAD) was developed for the simultaneous determination of canthaxanthin and astaxanthin in egg yolks.
Samples were extracted with acetonitrile in ultrasonic bath for 20 minutes and then purified by freezing-lipid filtration and solid phase extraction (SPE). After being vaporized to dryness by nitrogen blowing and made up to volume with methanol, the extract solution was chromatographically separated in C18 column with a unitary mobile phase consisting of acetonitrile. The proposed method was validated in terms of linearity, precision, accuracy, and limit of detection (LOD).
Regression analysis revealed a good linearity between peak area of each analyte and its concentration (r > or = 0.998). The intra- and inter-day relative standard deviations (RSDs) were less than 3.6% and 5.2%, respectively. LODs of canthaxanthin and astaxanthin were 0.035 and 0.027 microg/mL (S/N = 3). The average recoveries of canthaxanthin and astaxanthin were 91.5% and 88.7%.
The proposed method is simple, fast and easy to apply.
建立一种反相高效液相色谱（RP-HPLC）结合二极管阵列检测器（DAD）同时测定蛋黄中角黄素和虾青素的方法。
样品用乙腈在超声浴中提取20分钟，然后通过冷冻脂质过滤和固相萃取（SPE）进行纯化。经氮气吹干至干并以甲醇定容后，提取液在C18柱上用由乙腈组成的单一流动相进行色谱分离。该方法在线性、精密度、准确度和检测限（LOD）方面进行了验证。
回归分析表明，各分析物的峰面积与其浓度之间具有良好的线性关系（r≥0.998）。日内和日间相对标准偏差（RSD）分别小于3.6%和5.2%。角黄素和虾青素的检测限分别为0.035和0.027μg/mL（S/N = 3）。角黄素和虾青素的平均回收率分别为91.5%和88.7%。
该方法简单、快速且易于应用。
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