Pires Marcos M, Chmielewski Jean
Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, Indiana 47907-2084, USA.
Org Lett. 2008 Mar 6;10(5):837-40. doi: 10.1021/ol702769n. Epub 2008 Feb 8.
Glutathione is a crucial component of the redox homeostasis of cells, and altered levels have been linked to human pathologies. We constructed a latent fluorophore (RhoSS) that responds to cellular thiols in vitro and in cyto following intracellular reduction by glutathione to yield rhodamine 110. Importantly, RhoSS was demonstrated to respond to changing levels of glutathione in cells. This compound represents a class of rationally designed latent fluorophores with exciting potential for monitoring cellular thiols.
谷胱甘肽是细胞氧化还原稳态的关键组成部分，其水平的改变与人类疾病有关。我们构建了一种潜在荧光团（RhoSS），它在体外和细胞内被谷胱甘肽还原后能对细胞硫醇作出反应，产生罗丹明110。重要的是，RhoSS被证明能对细胞内谷胱甘肽水平的变化作出反应。这种化合物代表了一类经过合理设计的潜在荧光团，在监测细胞硫醇方面具有令人兴奋的潜力。
