Xie J, Bian H, Qi S, Xu Y, Tang J, Li T, Liu X
Burns Department, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, PR China.
Clin Exp Dermatol. 2008 Mar;33(2):176-82. doi: 10.1111/j.1365-2230.2007.02573.x.
Basic fibroblast growth factor (bFGF) has shown potential in clinical practice to accelerate wound healing, but the underlying biomolecular mechanism remains largely unknown. Fibroblasts are the most important cells involved in producing and remodelling the extracellular matrix (ECM) in wound healing, and are one of the major target cells of bFGF in wound repair. To date, however, we have little idea of whether there is any specific relationship between bFGF and ECM metabolism. This study aimed to investigate whether bFGF improves wound repair by regulating the balance of ECM synthesis and degradation.
To investigate the effects of bFGF on the expression of fibronectin, collagen and matrix metalloproteinase (MMP)-1 of human skin fibroblasts (HSFs) and to evaluate whether it contributes to improving the quality of wound healing.
HSFs were stimulated with bFGF for 72 h, and then production of fibronectin, collagen and MMP-1 was detected, using reverse transcription PCR at the transcriptional level and Western blot analysis at post-transcriptional level.
bFGF stimulation resulted in increases in fibronectin expression of 1.31-, 1.47-, 1.57- and 1.62-fold in a dose-dependent manner in response to 10 ng/mL, 50 ng/mL, 100 ng/mL and 500 ng/mL of bFGF, respectively, but had no effect on the expression of collagen. Further investigation revealed that bFGF dose-dependently upregulated the expression of MMP-1.
This study supports the hypothesis that bFGF has the potential to accelerate wound healing and improve the quality of wound healing by regulating the balance of ECM synthesis and degradation, suggesting a potential antiscarring role in wound healing.
碱性成纤维细胞生长因子（bFGF）在临床实践中已显示出加速伤口愈合的潜力，但其潜在的生物分子机制仍 largely 未知。成纤维细胞是伤口愈合过程中参与产生和重塑细胞外基质（ECM）的最重要细胞，并且是伤口修复中 bFGF 的主要靶细胞之一。然而，迄今为止，我们对 bFGF 与 ECM 代谢之间是否存在任何特定关系知之甚少。本研究旨在调查 bFGF 是否通过调节 ECM 合成与降解的平衡来改善伤口修复。
研究 bFGF 对人皮肤成纤维细胞（HSFs）中纤连蛋白、胶原蛋白和基质金属蛋白酶（MMP）-1 表达的影响，并评估其是否有助于提高伤口愈合质量。
用 bFGF 刺激 HSFs 72 小时，然后在转录水平使用逆转录 PCR 以及在转录后水平使用蛋白质印迹分析检测纤连蛋白、胶原蛋白和 MMP-1 的产生。
bFGF 刺激导致在分别响应 10 ng/mL、50 ng/mL、100 ng/mL 和 500 ng/mL 的 bFGF 时，纤连蛋白表达以剂量依赖性方式分别增加 1.31 倍、1.47 倍、1.57 倍和 1.62 倍，但对胶原蛋白的表达没有影响。进一步研究表明，bFGF 剂量依赖性地上调 MMP-1 的表达。
本研究支持以下假设，即 bFGF 有潜力通过调节 ECM 合成与降解的平衡来加速伤口愈合并提高伤口愈合质量，表明其在伤口愈合中具有潜在的抗瘢痕形成作用。
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