Roura Elena, Andrés-Lacueva Cristina, Estruch Ramon, Lourdes Mata Bilbao M, Izquierdo-Pulido Maria, Lamuela-Raventós Rosa M
Department of Nutrition and Food Science-CeRTA, University of Barcelona, Av/ Joan XXIII s/n, Barcelona 08028, Spain.
Br J Nutr. 2008 Oct;100(4):846-51. doi: 10.1017/S0007114508922534. Epub 2008 Feb 7.
The effect of different food matrices on the metabolism and excretion of polyphenols is uncertain. The objective of the study was to evaluate the possible effect of milk on the excretion of (2)-epicatechin metabolites from cocoa powder after its ingestion with and without milk. Twenty-one volunteers received the following three test meals each in a randomised cross-over design with a 1-week interval between meals: (1) 250 ml whole milk as a control; (2) 40 g cocoa powder dissolved in 250 ml whole milk (CC-M); (3) 40 g cocoa powder dissolved in 250 ml water (CC-W). Urine was collected before consumption and during the 0-6, 6-12 and 12-24 h periods after consumption. (2)-Epicatechin metabolite excretion was measured using liquid chromatography-MS. One (2)-epicatechin glucuronide and three (2)-epicatechin sulfates were detected in urine excreted after the intake of the two cocoa beverages (CC-M and CC-W). The results show that milk does not significantly affect the total amount of metabolites excreted in urine. However, differences in metabolite excretion profiles were observed; there were changes in the glucuronide and sulfate excretion rates, and the sulfation position between the period of excretion and the matrix. The matrix in which polyphenols are consumed can affect their metabolism and excretion, and this may affect their biological activity. Thus, more studies are needed to evaluate the effect of these different metabolite profiles on the body.
不同食物基质对多酚代谢和排泄的影响尚不确定。本研究的目的是评估牛奶对摄入含和不含牛奶的可可粉后（+）-表儿茶素代谢物排泄的可能影响。21名志愿者采用随机交叉设计，每餐间隔1周，接受以下三种测试餐：（1）250毫升全脂牛奶作为对照；（2）40克可可粉溶解于250毫升全脂牛奶中（CC-M）；（3）40克可可粉溶解于250毫升水中（CC-W）。在食用前以及食用后的0 - 6、6 - 12和12 - 24小时期间收集尿液。使用液相色谱 - 质谱法测量（+）-表儿茶素代谢物的排泄情况。在摄入两种可可饮料（CC-M和CC-W）后排出的尿液中检测到一种（+）-表儿茶素葡萄糖醛酸苷和三种（+）-表儿茶素硫酸盐。结果表明，牛奶对尿液中排泄的代谢物总量没有显著影响。然而，观察到代谢物排泄谱存在差异；葡萄糖醛酸苷和硫酸盐的排泄率以及排泄期间和基质之间的硫酸化位置发生了变化。食用多酚的基质会影响它们的代谢和排泄，这可能会影响它们的生物活性。因此，需要更多研究来评估这些不同代谢物谱对身体的影响。
