Roura Elena, Almajano Ma Pilar, Bilbao Ma Lourdes Mata, Andrés-Lacueva Cristina, Estruch Ramon, Lamuela-Raventós Rosa Ma
Department of Nutrition and Food Science, University of Barcelona, Barcelona, Spain.
Free Radic Res. 2007 Aug;41(8):943-9. doi: 10.1080/10715760701435236.
Associations between cocoa consumption in humans, excreted metabolites and total antioxidant capacity (TAC) have been scarcely investigated. The aims of the study were to investigate the epicatechin (( - )-Ec) metabolites excreted in urine samples after an intake of 40 g of cocoa powder along with the TAC of these urine samples and the relation between both the analyses. Each of the 21 volunteers received two interventions, one with a polyphenol-rich food (PRF) and one with a polyphenol-free food (PFF) in a randomized cross-over study. Urine samples were taken before and during 24 h at 0-6, 6-12 and 12-24 h periods after test intake. The excreted ( - )-Ec metabolites and the TAC were determined in urine samples by LC-MS/MS and TEAC assay, respectively. The maximum excretion of ( - )-Ec metabolites and the maximum TAC value were observed in urine samples excreted between 6 and 12 h after PRF consumption. Significance of TAC increase was found in urine samples excreted during 0-6 and 6-12 h (66.6 and 72.67%, respectively, with respect to the 0 h).
人类食用可可、排泄代谢物与总抗氧化能力（TAC）之间的关联鲜有研究。本研究旨在调查摄入40克可可粉后尿样中排泄的表儿茶素（（-）-Ec）代谢物以及这些尿样的TAC，以及两项分析之间的关系。在一项随机交叉研究中，21名志愿者每人接受两种干预，一种是富含多酚的食物（PRF），另一种是不含多酚的食物（PFF）。在测试摄入后的0 - 6、6 - 12和12 - 24小时期间，于24小时内的不同时间点采集尿样。分别通过液相色谱 - 串联质谱法（LC - MS/MS）和总抗氧化能力（TEAC）测定法测定尿样中排泄的（-）-Ec代谢物和TAC。在食用PRF后6至12小时排泄的尿样中观察到（-）-Ec代谢物的最大排泄量和TAC的最大值。在0 - 6小时和6 - 12小时排泄的尿样中发现TAC增加具有显著性（相对于0小时，分别为66.6%和72.67%）。
