Bartels Mette Damkjaer, Boye Kit, Rhod Larsen Anders, Skov Robert, Westh Henrik
Hvidovre Hospital, Hvidovre, Denmark.
Emerg Infect Dis. 2007 Oct;13(10):1533-40. doi: 10.3201/eid1310.070503.
In Copenhagen, methicillin-resistant Staphylococcus aureus (MRSA) accounted for <15 isolates per year during 1980-2002. However, since 2003 an epidemic increase has been observed, with 33 MRSA cases in 2003 and 110 in 2004. We analyzed these 143 cases epidemiologically and characterized isolates by pulsed-field gel electrophoresis, Staphylococcus protein A (spa) typing, multilocus sequence typing, staphylococcal chromosome cassette (SCC) mec typing, and detection of Panton-Valentine leukocidin (PVL) genes. Seventy-one percent of cases were community-onset MRSA (CO-MRSA); of these, 36% had no identified risk factors. We identified 29 spa types (t) and 16 sequence types (STs) belonging to 8 clonal complexes and 3 ST singletons. The most common clonal types were t024/ST8-IV, t019/ST30-IV, t044/ST80-IV, and t008/ST8-IV (USA300). A total of 86% of isolates harbored SCCmec IV, and 44% had PVL. Skin and soft tissue infections dominated. CO-MRSA with diverse genetic backgrounds is rapidly emerging in a low MRSA prevalence area.
在哥本哈根，1980年至2002年期间，耐甲氧西林金黄色葡萄球菌（MRSA）每年分离株数少于15株。然而，自2003年以来，出现了流行趋势，2003年有33例MRSA病例，2004年有110例。我们对这143例病例进行了流行病学分析，并通过脉冲场凝胶电泳、葡萄球菌蛋白A（spa）分型、多位点序列分型、葡萄球菌染色体盒（SCC）mec分型以及检测杀白细胞素（PVL）基因对分离株进行了特征分析。71%的病例为社区获得性MRSA（CO-MRSA）；其中36%未发现危险因素。我们鉴定出29种spa型（t）和16种序列型（STs），它们属于8个克隆复合体和3个单序列型。最常见的克隆类型为t024/ST8-IV、t019/ST30-IV、t044/ST80-IV和t008/ST8-IV（USA300）。总共86% 的分离株携带SCCmec IV，44% 携带PVL基因。皮肤和软组织感染占主导。在MRSA低流行地区，具有不同遗传背景的CO-MRSA正在迅速出现。
Zotero 插件