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海湾蟾鱼(Opsanus tau)内耳中内淋巴和外淋巴的离子组成。

Ionic composition of endolymph and perilymph in the inner ear of the oyster toadfish, Opsanus tau.

作者信息

Ghanem Tamer A, Breneman Kathryn D, Rabbitt Richard D, Brown H Mack

机构信息

Department of Bioengineering, University of Utah, Salt Lake City, Utah 84112, USA.

出版信息

Biol Bull. 2008 Feb;214(1):83-90. doi: 10.2307/25066662.

Abstract

The concentrations of free Na+, K+, Ca(+, and Cl(-)in endolymph and perilymph from the inner ear of the oyster toadfish, Opsanus tau, were measured in vivo using double-barreled ion-selective electrodes. Perilymph concentrations were similar to those measured in other species, while endolymph concentrations were similar to those measured previously in elasmobranch fish, though significantly different from concentrations reported in mammals. Perilymph concentrations (mean +/- std. dev.) were as follows: Na+, 129 mmol l(-1) +/- 20; K+, 4.96 mmol l(-1) +/- 2.67; Ca2+, 1.83 mmol l(-1) +/- 0.27; and Cl(-), 171 mmol l(-1) +/- 20. Saccular endolymph concentrations were Na+, 166 mmol l(-1) +/- 22; K+, 51.4 mmol l(-1) +/- 16.7; Ca2+, 2.88 mmol l(-1) +/- 0.27; and Cl(-), 170 mmol l(-1) +/- 12; and semicircular canal (utricular vestibule) endolymph concentrations were Na+, 122 mmol l(-1) +/- 15; K+, 47.7 mmol l(-1) +/- 13.2; Ca2+, 1.78 mmol l(-1) +/- 0.48; Cl(-), 176 mmol l(-1) +/- 27. The relatively high concentrations of Ca2+ and Na+ in the endolymph may have significant implications for the physiological function of the mechanoelectrical transduction channels in the vestibular hair cells of fish compared to those of their mammalian counterparts.

摘要

使用双管离子选择性电极在体内测量了海湾蟾鱼(Opsanus tau)内耳内淋巴和外淋巴中游离Na⁺、K⁺、Ca²⁺和Cl⁻的浓度。外淋巴浓度与在其他物种中测得的浓度相似,而内淋巴浓度与先前在软骨鱼类中测得的浓度相似,尽管与哺乳动物中报道的浓度有显著差异。外淋巴浓度(平均值±标准差)如下:Na⁺,129 mmol·l⁻¹±20;K⁺,4.96 mmol·l⁻¹±2.67;Ca²⁺,1.83 mmol·l⁻¹±0.27;Cl⁻,171 mmol·l⁻¹±20。球囊内淋巴浓度为:Na⁺,166 mmol·l⁻¹±22;K⁺,51.4 mmol·l⁻¹±16.7;Ca²⁺,2.88 mmol·l⁻¹±0.27;Cl⁻,170 mmol·l⁻¹±12;半规管(椭圆囊前庭)内淋巴浓度为:Na⁺,122 mmol·l⁻¹±15;K⁺,47.7 mmol·l⁻¹±13.2;Ca²⁺,1.78 mmol·l⁻¹±0.48;Cl⁻,176 mmol·l⁻¹±27。与哺乳动物相比,内淋巴中相对较高的Ca²⁺和Na⁺浓度可能对鱼类前庭毛细胞中机械电转导通道的生理功能有重要影响。

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