Zharkov D O
Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
Cell Mol Life Sci. 2008 May;65(10):1544-65. doi: 10.1007/s00018-008-7543-2.
DNA repair is a collection of several multienzyme, multistep processes keeping the cellular genome intact against genotoxic insults. One of these processes is base excision repair, which deals with the most ubiquitous lesions in DNA: oxidative base damage, alkylation, deamination, sites of base loss and single-strand breaks, etc. Individual enzymes acting in base excision repair have been identified. The recent years were marked with many advances in understanding of their structure and many interactions that make base excision repair a functional, versatile system. This review describes the current knowledge of structural biology and biochemistry of individual steps of base excision repair, several subpathways of the common base excision repair pathway, and interactions of the repair process with other cellular processes.
DNA修复是一系列多酶、多步骤的过程，可保护细胞基因组免受基因毒性损伤。其中一个过程是碱基切除修复，它处理DNA中最普遍存在的损伤：氧化碱基损伤、烷基化、脱氨基、碱基缺失位点和单链断裂等。已经鉴定出参与碱基切除修复的各个酶。近年来，在理解它们的结构以及使碱基切除修复成为一个功能性、通用系统的许多相互作用方面取得了许多进展。本综述描述了碱基切除修复各个步骤的结构生物学和生物化学的当前知识、常见碱基切除修复途径的几个子途径，以及修复过程与其他细胞过程的相互作用。
