Pap Károly, Berki Sándor, Shisha Tamás, Kiss Sándor, Szoke György
Department of Orthopaedics, Semmelweis University of Budapest, Budapest, Hungary.
Int Orthop. 2009 Apr;33(2):561-6. doi: 10.1007/s00264-008-0514-2. Epub 2008 Feb 8.
This study evaluated the histological changes in muscle tissue after limb lengthening in skeletally mature and immature rabbits and assessed the most vulnerable level of striated muscle. Twenty-three male domestic white rabbits, divided into six groups, were operated on and different lengthening protocols were used in the mature and immature rabbits. The histopathological changes were analysed by a semi-quantitative method according to the scoring system of Lee et al. (Acta Orthop Scand 64(6):688-692, 1993). After the evaluation of the five main degenerative parameters (muscle atrophy, muscle nuclei internalisation, degeneration of the muscle fibre, perimysial and endomysial fibrosis, haematomas), it is evident that the adults lengthened at a rate of 1.6 mm/day showed more degenerative changes than those lengthened at 0.8 mm/day. The adult 1.6 mm/day lengthened group presented significantly higher damage in the muscle and lower regenerative signs compared with the young 1.6 mm/day lengthened group, according to the summarised degenerative scores.
本研究评估了骨骼成熟和未成熟兔子肢体延长后肌肉组织的组织学变化，并评估了横纹肌最易受损的水平。将23只雄性家养白兔分为6组，对其进行手术，并在成熟和未成熟兔子中采用不同的延长方案。根据Lee等人（《斯堪的纳维亚矫形外科学杂志》64(6):688 - 692, 1993）的评分系统，采用半定量方法分析组织病理学变化。在评估五个主要退行性参数（肌肉萎缩、肌核内化、肌纤维变性、肌束膜和肌内膜纤维化、血肿）后，很明显，以1.6毫米/天的速度延长的成年兔子比以0.8毫米/天的速度延长的兔子表现出更多的退行性变化。根据汇总的退行性评分，成年1.6毫米/天延长组与幼年1.6毫米/天延长组相比，肌肉损伤明显更高，再生迹象更低。
