锥虫H2Bv在富含H3 K4和K76三甲基化的核小体中取代H2B。
Trypanosome H2Bv replaces H2B in nucleosomes enriched for H3 K4 and K76 trimethylation.
作者信息
Mandava Veena, Janzen Christian J, Cross George A M
机构信息
Laboratory of Molecular Parasitology, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA.
出版信息
Biochem Biophys Res Commun. 2008 Apr 18;368(4):846-51. doi: 10.1016/j.bbrc.2008.01.144. Epub 2008 Feb 7.
Abstract
Some inroads have been made into characterizing histone variants and post translational modifications of histones in Trypanosoma brucei. Histone variant H2BV lysine 129 is homologous to Saccharomyces cerevisiae H2B lysine 123, whose ubiquitination is required for methylation of H3 lysines 4 and 79. We show that T. brucei H2BV K129 is not ubiquitinated, but trimethylation of H3 K4 and K76, homologs of H3 K4 and K79 in yeast, was enriched in nucleosomes containing H2BV. Mutation of H2BV K129 to alanine or arginine did not disrupt H3 K4 or K76 methylation. These data suggest that H3 K4 and K76 methylation in trypanosomes is regulated by a novel mechanism, possibly involving the replacement of H2B with H2BV in the nucleosome.
摘要
在布氏锥虫中，对组蛋白变体和组蛋白翻译后修饰的表征已经取得了一些进展。组蛋白变体H2BV赖氨酸129与酿酒酵母H2B赖氨酸123同源，后者的泛素化是H3赖氨酸4和79甲基化所必需的。我们发现布氏锥虫H2BV K129没有被泛素化，但酵母中H3 K4和K79的同源物H3 K4和K76的三甲基化在含有H2BV的核小体中富集。将H2BV K129突变为丙氨酸或精氨酸不会破坏H3 K4或K76的甲基化。这些数据表明，锥虫中H3 K4和K76的甲基化受一种新机制调控，可能涉及核小体中H2B被H2BV取代。
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