氟脱氧尿苷同步化在急性淋巴细胞白血病细胞遗传学研究中的应用。

The use of fluorodeoxyuridine synchronization for cytogenetic investigation of acute lymphoblastic leukemia.

作者信息

Garipidou V, Secker-Walker L M

机构信息

Department of Haematology, Royal Free Hospital and School of Medicine, London, U.K.

出版信息

Cancer Genet Cytogenet. 1991 Mar;52(1):107-11. doi: 10.1016/0165-4608(91)90060-8.

DOI:10.1016/0165-4608(91)90060-8

PMID:1826229

Abstract

The role of fluorodeoxyuridine (FUdR) synchronization in cytogenetic analysis of acute lymphoblastic leukemia (ALL) was investigated using samples of bone marrow (BM) (10 patients) and peripheral blood (PB) (2 patients), prepared for chromosome analysis using both 24-hour unstimulated cultures (24-hr) and cultures synchronized with FUdR. The mitotic index (MI) in FUdR was lower than in 24-hr in 8 of 10 BM and 2 of 2 PB cultures. The quality of the metaphases was the same in both cultures. The FUdR had a lower percentage of abnormal cells than the 24-hr in the 7 BM samples with a normal/abnormal population and sufficient analyzable cells in each culture for comparison (p less than 0.05). PB FUdR cultures yielded only normal cells. We conclude that FUdR cultures are inferior to 24-hr cultures for chromosome analysis in ALL.

摘要

使用10例骨髓（BM）样本和2例外周血（PB）样本，对氟脱氧尿苷（FUdR）同步化在急性淋巴细胞白血病（ALL）细胞遗传学分析中的作用进行了研究，这些样本采用24小时非刺激培养（24小时）和FUdR同步化培养两种方式制备用于染色体分析。在10例BM培养物中的8例以及2例PB培养物中的2例中，FUdR培养中的有丝分裂指数（MI）低于24小时培养。两种培养中中期分裂相的质量相同。在7例具有正常/异常细胞群体且每种培养中有足够可分析细胞用于比较的BM样本中，FUdR培养中异常细胞的百分比低于24小时培养（p小于0.05）。PB的FUdR培养仅产生正常细胞。我们得出结论，在ALL的染色体分析中，FUdR培养不如24小时培养。