Saint-Ruf C, Malfoy B, Scholl S, Zafrani B, Dutrillaux B
URA 620 CNRS, Institut Curie, Paris, France.
Oncogene. 1991 Mar;6(3):403-6.
The glutathione S-transferase gene (GST pi) is located on the same chromosome band (11q13) as proto-oncogenes INT2 and HSTF1 which are frequently amplified in breast cancer. Using the Southern blot technique, we looked for the amplification of the GST pi gene in 17 fresh tumors from human mammary carcinoma. The tumors were preselected because either they had an amplification of the INT2 proto-oncogene detected by dot blot, or their karyotypes exhibited or did not exhibit homogeneously staining regions, a cytogenetic character indicating amplification. Coamplification of GST pi, HSTF1 and INT2 was observed in five tumors, and coamplification of GST pi and HSTF1 without amplification of INT2 in another tumor. We also observed coamplification of GST pi, INT2, HSTF1 in the mammary carcinoma cell line MDA/MB134, whereas GST pi alone was amplified in the mammary epithelial cell line HBL100. These results indicate that INT2, HSTF1 and GST pi belong to the same large amplicon. Since GST pi is involved in intracellular detoxication and since chemotherapeutic drugs are among its substrates, it will be of interest to study GST pi gene expression as well as the response to chemotherapy in patients presenting this amplicon.
谷胱甘肽S-转移酶基因（GST pi）与原癌基因INT2和HSTF1位于同一染色体带（11q13）上，而这两个原癌基因在乳腺癌中经常发生扩增。我们运用Southern印迹技术，在17例人乳腺癌新鲜肿瘤中探寻GST pi基因的扩增情况。这些肿瘤是经过预先筛选的，因为要么通过斑点印迹检测到它们存在INT2原癌基因的扩增，要么其核型显示或未显示均匀染色区，这是一种表明扩增的细胞遗传学特征。在5例肿瘤中观察到GST pi、HSTF1和INT2的共同扩增，在另一例肿瘤中观察到GST pi和HSTF1的共同扩增但INT2未扩增。我们还在乳腺癌细胞系MDA/MB134中观察到GST pi、INT2、HSTF1的共同扩增，而在乳腺上皮细胞系HBL100中仅GST pi被扩增。这些结果表明INT2、HSTF1和GST pi属于同一个大的扩增子。由于GST pi参与细胞内解毒，且化疗药物是其底物之一，研究具有该扩增子的患者中GST pi基因表达以及对化疗的反应将是有意义的。
