Lundblad Eirik W, Xiao Gaoping, Ko Jae-Hyeong, Altman Sidney
Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA.
Proc Natl Acad Sci U S A. 2008 Feb 19;105(7):2354-7. doi: 10.1073/pnas.0711977105. Epub 2008 Feb 8.
A method of inhibiting the expression of particular genes by using external guide sequences (EGSs) has been improved in its rapidity and specificity. Random EGSs that have 14-nt random sequences are used in the selection procedure for an EGS that attacks the mRNA for a gene in a particular location. A mixture of the random EGSs, the particular target RNA, and RNase P is used in the diagnostic procedure, which, after completion, is analyzed in a gel with suitable control lanes. Within a few hours, the procedure is complete. The action of EGSs designed by an older method is compared with EGSs designed by the random EGS method on mRNAs from two bacterial pathogens.
一种通过使用外部引导序列（EGS）来抑制特定基因表达的方法在速度和特异性方面得到了改进。在针对特定位置的基因的mRNA进行攻击的EGS的筛选过程中，使用了具有14个核苷酸随机序列的随机EGS。在诊断过程中使用随机EGS、特定靶RNA和核糖核酸酶P的混合物，完成后在带有合适对照泳道的凝胶中进行分析。几小时内即可完成该过程。将用较旧方法设计的EGS与用随机EGS方法设计的EGS对两种细菌病原体的mRNA的作用进行了比较。
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