Sýkora J, Zástĕra M
Institut hygieny a epidemiologie, Praha.
Cesk Epidemiol Mikrobiol Imunol. 1991 Jan;40(1):22-8.
The described model of experimental pneumocystosis is based on the induction of natural latent infection of P. carinii in Wistar strain laboratory rats. As to pharmacological inducing agents immunosuppresive preparations such as Hydrocortisone sol. inj. (Spofa), Cyclosporin A (Merck) and Dexamethasone (Spofa) were used whereby the latter was effective in up 50%. As to non-pharmacological inducing agents, the authors used in combination with the tested inducers a low protein diet (less than 8% protein in the diet); for suppression of associated bacterial contamination tetracycline was added to drinking water. From the infected lungs by two different methods two types of antigens were prepared 1) PCL antigen (Pneumocystis carinii lavage antigen) isolated by rinsing of the lungs and 2) PCWD antigen (P. carinii whole digest antigen) isolated by digestion of the lungs with collagenase and trypsin. Cysts of P. carinii were detected by staining according to Giemsa (staining of internal structures of nuclear cysts) and by modified staining with toluidine blue O (staining of the cyst wall). For isolation of the antigen and for detection of cysts a combination of the two described methods seems to be best.
所描述的实验性肺孢子菌病模型基于在Wistar品系实验大鼠中诱导卡氏肺孢子虫的自然潜伏感染。至于药理诱导剂，使用了诸如氢化可的松注射液（Spofa公司）、环孢素A（默克公司）和地塞米松（Spofa公司）等免疫抑制制剂，其中后者的有效率高达50%。至于非药理诱导剂，作者将低蛋白饮食（饮食中蛋白质含量低于8%）与受试诱导剂联合使用；为抑制相关细菌污染，在饮用水中添加了四环素。通过两种不同方法从受感染的肺中制备了两种抗原：1）通过冲洗肺分离得到的PCL抗原（卡氏肺孢子虫灌洗抗原）和2）通过用胶原酶和胰蛋白酶消化肺分离得到的PCWD抗原（卡氏肺孢子虫全消化抗原）。卡氏肺孢子虫囊肿通过吉姆萨染色（核囊肿内部结构染色）和改良的甲苯胺蓝O染色（囊肿壁染色）进行检测。对于抗原的分离和囊肿的检测，两种所述方法的组合似乎是最佳的。
