Furuta T, Fujita M, Machii K, Kobayashi K, Kojima S, Ueda K
Department of Parasitology, University of Tokyo, Japan.
Lab Anim Sci. 1993 Dec;43(6):551-6.
Spontaneously generated fatal pneumocystosis was found in a congenitally athymic nude (rnu/rnu) rat colony. Severe pulmonary pneumocystosis was seen in rnu/rnu rats, and 10(7) to 10(8) cysts per lung were detected. No histologic changes were seen nor were Pneumocystis carinii organisms detected in heterozygous rnu/+ rats. Indirect immunofluorescence revealed strong reactivity in the sera from rnu/+ rats with rat-P. carinii cysts (originated from Wistar strain), compared with mouse- or human P. carinii cysts. The cysts isolated from rnu/rnu rats reacted strongly with antirat-P. carinii rat serum in comparison with antimouse-P. carinii rabbit serum and serum from a human with pneumocystosis. Immunoblotting studies using rat-P. carinii antigen showed that 116-kDa antigen reacted with the antirat-P. carinii serum and sera from rnu/+ rats but not with the antimouse-P. carinii serum and the serum from a human with pneumocystosis, whereas 52-kDa antigens of rat-P. carinii were recognized by all the anti-P. carinii sera tested. On the basis of our findings, the outbreak was caused by the P. carinii indigenous to the rat.
在一个先天性无胸腺裸（rnu/rnu）大鼠群体中发现了自发产生的致命性肺孢子虫病。在rnu/rnu大鼠中可见严重的肺部肺孢子虫病，每只肺检测到10(7)至10(8)个囊肿。在杂合子rnu/+大鼠中未观察到组织学变化，也未检测到卡氏肺孢子虫生物体。间接免疫荧光显示，与小鼠或人卡氏肺孢子虫囊肿相比，rnu/+大鼠血清与源自Wistar品系的大鼠卡氏肺孢子虫囊肿有强烈反应。与抗小鼠卡氏肺孢子虫兔血清和患有肺孢子虫病的人的血清相比，从rnu/rnu大鼠分离的囊肿与抗大鼠卡氏肺孢子虫大鼠血清有强烈反应。使用大鼠卡氏肺孢子虫抗原的免疫印迹研究表明，116-kDa抗原与抗大鼠卡氏肺孢子虫血清和rnu/+大鼠血清反应，但不与抗小鼠卡氏肺孢子虫血清和患有肺孢子虫病的人的血清反应，而大鼠卡氏肺孢子虫的52-kDa抗原被所有测试的抗卡氏肺孢子虫血清识别。根据我们的研究结果，此次疫情是由大鼠自身携带的卡氏肺孢子虫引起的。
