通过高效液相色谱法对中性寡糖进行分级分离和分析。
Fractionation and analysis of neutral oligosaccharides by HPLC.
作者信息
Hayes B K, Varki A
机构信息
University of Alabama at Birmingham, Birmingham, Alabama, USA.
出版信息
Curr Protoc Mol Biol. 2001 May;Chapter 17:Unit17.21B. doi: 10.1002/0471142727.mb1721bs32.
Abstract
This unit describes the fractionation and analysis of neutral oligosaccharides by high-performance liquid chromatography (HPLC) on bonded amine columns. Separation is based upon hydrogen bonding between the NH2 groups of the column and the hydroxyl groups of the oligosaccharides. A support protocol describes the reduction and desalting of neutral oligosaccharides with sodium borohydride.
摘要
本单元介绍了通过键合胺柱上的高效液相色谱（HPLC）对中性寡糖进行分级分离和分析的方法。分离基于柱上NH2基团与寡糖羟基之间的氢键作用。一份支持性方案描述了用硼氢化钠对中性寡糖进行还原和脱盐的方法。
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