Derisi J
University of California, San Francisco, San Francisco, California, USA.
Curr Protoc Mol Biol. 2001 May;Chapter 22:Unit 22.1. doi: 10.1002/0471142727.mb2201s49.
Nucleic acid array technology refers to the use and fabrication of arrays containing thousands of nucleic acid samples bound to solid substrates such as glass microscope slides or silicon wafers. Because the physical area occupied by each sample is usually 50 to 200 mum in diameter, it is possible to assay nucleic acid samples representing entire genomes, ranging in size from 3,000 to 32,000 genes, on a single slide. Microarrays are good for, among other things, analyzing gene expression patterns, genotyping and genetic mapping, comparative genomic hybridization, polysome analysis, and DNA-protein interactions. This overview describes the technology and the uses, and provides valuable web site listings for readers to obtain additional information.
核酸阵列技术是指使用和制造包含数千个与固体基质（如玻璃显微镜载玻片或硅片）结合的核酸样本的阵列。由于每个样本所占据的物理区域直径通常为50至200微米，因此有可能在一张载玻片上分析代表整个基因组的核酸样本，这些基因组大小从3000到32000个基因不等。微阵列除其他用途外，还擅长分析基因表达模式、基因分型和基因图谱绘制、比较基因组杂交、多核糖体分析以及DNA-蛋白质相互作用。本概述介绍了该技术及其用途，并为读者提供了有价值的网站列表，以便获取更多信息。
